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Paraoxonase activity and antibodies to oxidized-LDL in chronic renal failure patients on renal replacement therapy

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Abstract

Serum paraoxonase (PON1) and antibodies to oxidized-LDL (anti ox-LDL) were measured in chronic renal failure subjects on renal replacement therapy such as hemodialysis (HD) peritoneal dialysis (PD) and transplantation (Txp). Paraoxonase activity was significantly lower in HD and PD group (P<0.001) than in control subjects. In transplant patients, paraoxonase activity was not significantly different from that of controls. Antibodies to ox-LDL was significantly higher in HD, PD and Transplant patients (P<0.0001) compared to control subjects. High titers of antibodies were observed in the HD group compared to the PD and Transplant subjects. A decrease in paraoxonase activity and high titers of Antibodies to ox-LDL in the dialysis group suggest a decreased cardio protective effect of HDL and enhanced risk of premature cardiovascular complications. Whereas in case of transplant subjects, there seems to be restoration of PON1 activity, but elevated levels of anti-oxLDL could still be a potential atherogenic factor. Hence, we propose that estimation of these two parameters can be used as a useful index to measure the cardiac risk in the above patient category

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References

  1. Durrington, P.N., Arrol, S. and Mackness, M.I. (1991) Paraoxonase prevents accumulation of lipoperoxidase in low-density lipoprotein.FEBS Lett. 286, 152–154.

    Article  PubMed  Google Scholar 

  2. Mackness, M.I., Abbott, C.A., Arrol, S. and Durrington, P.N. (1993) The role of High density lipoprotein and lipidsoluble antioxidant vitamins in inhibiting low-density lipoprotein oxidation.Biochem. J. 294, 829–835

    PubMed  CAS  Google Scholar 

  3. Watson, A.D., Berliner, J.A., Hama, S.Y., La Du, B.N., Faull, K.F., Fogelman, A.M. and Navab, M. (1995) Protective effect of high density lipoprotein associated paraoxonase.J. Clin. Invest. 96, 2882–2891.

    Article  PubMed  CAS  Google Scholar 

  4. Schiavon, R., De Fanti, E., Giavarina, D., Biasioli, S., Cavalcanti, G. and Guidi, G. (1996) Serum paraoxonase activity decreased in uremic patients.Clin. Chim. Acta. 247, 71–80

    Article  PubMed  CAS  Google Scholar 

  5. Gyorgy, P., Laszlo, A., Iidiko, S., Zoltan, B., Lajos, L. and Gyorgy, K. (1991) Serum paraoxonase activity changes in uremic and kidney transplanted patients.Nephron 83, 126–131

    Google Scholar 

  6. Witztum, J.L. and Steinberg, D. (1991) Role of oxidized low-density lipoprotein in atherogenesis.J. Clin. Invest. 88, 1785–92

    Article  PubMed  CAS  Google Scholar 

  7. Maggi, E., Bellazzi, R., Falaschi, F., Frattoni, A. and Perani, G., et al. (1994) Enhanced LDL oxidation in uremic patients: an additional mechanism for accelerated atherosclerosis?.Kidney Int. 45, 876–83

    Article  PubMed  CAS  Google Scholar 

  8. Haagen, L. and Brock, A. (1992) A new automated method for phenotyping arylesterase based upon inhibition of enzymatic hydrolysis of 4-nitrophenlyacetate by phenyl acetate.Eur. J. Clin. Chem. Clin. Biochem. 30, 391–395

    PubMed  CAS  Google Scholar 

  9. Chait, A., Brazg, R., Tribble, D. and Krauss, R. (1993) Susceptibility of small, dense, low-Density Lipoproteins to oxidative modification in subjects with the atherogenic lipoprotein phenotype, pattern B.Am. J. Med. 94, 350–356

    Article  PubMed  CAS  Google Scholar 

  10. Aviram, M., Rosenblat, M. and Billecke, S. et al (1999) Human serum paraoxonase (PON1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants.Free Radic. Biol. Med. 26, 892–904

    Article  PubMed  CAS  Google Scholar 

  11. Dantoine, T.F., Debord, J., Charmes, J.P. et al. (1998) Decrease of serum paraoxonase activity in chronic renal failure.J. Am. Soc. Nephrol. 9, 2082–2088.

    PubMed  CAS  Google Scholar 

  12. Paul, J.L., Sall, N.D., Soni, et al. (1993) Lipid peroxidation abnormalities in hemodialysed patients.Nephron 64, 106–109

    Article  PubMed  CAS  Google Scholar 

  13. Cristol, J.P., Canaud, B., Rabesandratana, H., Gaillard, I., Serre, A. and Mion, C (1994) Enhancement of reactive oxygen species production and cell surface markers expression during hemodialysis.Nephrol. Dial. Transplant 9, 389–394.

    PubMed  CAS  Google Scholar 

  14. Himmelfarl, J., Lazarus, M. and Hakim, R. (1991) Reactive oxygen species production by monocytes and polymorphonuclear leukocytes during dialysis.Am. J. Kidney Dis. 17, 271–276

    Google Scholar 

  15. Odetti, P., Garibaldi, S., Gurreri, G., et al (1996) Protein oxidation in hemodialysis and kidney transplantation.Metabolism 45, 1319

    Article  PubMed  CAS  Google Scholar 

  16. Cristol, J.P., Maggi, M.F., Vela, C., Descomps, B. and Mourad, G. (1996) Lipid metabolism and oxidative stress in renal transplantation: implications for chronic rejection.Transplant Proc. 28 (5),2820.

    PubMed  CAS  Google Scholar 

  17. Inselmann, G., Blank, M. and Baumann, K. (1988) Cyclosporine A induces lipid peroxidation in microsomes and effects on active and passive glucose transport by brush border membrane vesicles of rat kidney.Res. Commun. Chem. Pathol. Pharmacol. 62, 207.

    PubMed  CAS  Google Scholar 

  18. Serino, F., Grevel, J., Napoli, K.L., Kahan, B.D. and Strobel, H.W. (1994) Oxygen radical formation by the cytochrome p 450 system as a cellular mechanism for cyclosporine toxicity.Transplant Proc., 26, 2916.

    PubMed  CAS  Google Scholar 

  19. Wang, C. and Salahudeen, A.K. (1995) Lipid peroxidation accompanies cyclosporine nephrotoxicity: Effects of vitamin E.Kidney Int. 47, 917–934.

    Article  Google Scholar 

  20. Steinberg, D., Parthasarathy, S., Carew, T.E., Khoo, M.C., Witztum, J.L. and Beyong, C. (1989) Modifications of lowdensity lipoprotein that increase its atherogenicity.N. Engl. J. Med. 320, 915–924.

    PubMed  CAS  Google Scholar 

  21. Esterbauer, H., Gebicki, J., Puhl, H. and Jurgens, G. (1992) The role of lipid peroxidation and antioxidants in oxidative modification of LDL.Free Radic. Biol. Med. 13, 341–390.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemisitry, Kasturba Medical College, Manipal, Kamataka

    Anjali Rao

  2. Manipal Hospital, No. 98, Rustom bagh, Airport Road, 560 003, Banglore, Karanata

    P. R. Krishnaswamy, Murali W. (Senior clinical Biochemist) & H. Sudarshan Ballal

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  1. P. R. Krishnaswamy
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  2. Anjali Rao
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Correspondence to Murali W..

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Krishnaswamy, P.R., Rao, A., Murali, W. et al. Paraoxonase activity and antibodies to oxidized-LDL in chronic renal failure patients on renal replacement therapy. Indian J Clin Biochem 21, 173–176 (2006). https://doi.org/10.1007/BF02912937

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