Abstract
Dependence of the broth effeot and the phenomenon of mutation frequency decline on dose of the applied UV radiation was investigated in the strainEscherichia coli B/r Hcr+ thy trp. Reversions to Trp+ were followed. The degree of the broth effect and the mutation frequency decline is minimal within the range of UV doses corresponding to a survival of cells lower than 10-1. In connection with the two effects, excision of thymine dimers, initiation of synthesis, synthesis and degradation of DNA were also investigated. It was found that stimulation or inhibition of an inaccurate postreplication repair mechanism, rather than inhibition or stimulation of excision of thymine dimers, are responsible for the broth effect and the mutation frequency decline, respectively.
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Balgavý P.: Effect of potassium nitrate on photoreaotivation of Escherichis coli cells.Z. Naturforsch.28c, 757 (1973).
Balgavý P., Mašek F., Turek R.: Thymine dimer excision and DNA degradation after thymine starvation and ultraviolet irradiation inEscherichia coli B/r Hcr+.Biochim. Biophys. Acta 390, 24 (1975).
Balgavý P., Rauko P.: Elimination of lethal and pre-mutational lesions during the photoreactivation of UV-irradiatedEscherichia coli.Folia Microbiol. 21, 1 (1976a).
Balgavý P., Rauko P.: Role of post-replication and excision repair mechanism in the induction of Trp+ revertants of UV irradiatedEscherichia coli.Folia Microbiol. 21, 90 (1976b).
Balgavý P., Turek R.: Inhibition of excision repair without influence upon UV-sensitivity and UV-mutability inEscherichia coli B/r Hcr+.Z. Naturforsch. 30c, 406 (1975).
Bockrath R. C., Cheung M. K.: The role of nutrient broth supplementation in UV mutagenesis ofE. coli.Mutation Res. 19, 23 (1973).
Bridges B. A., Dennis R. E., Munson R. J.: Differential induction and repair of ultraviolet damage leading to true reversions and external supressor mutations of an ochre codon inEscherichia coli B/r WP2.Genetics 57, 897 (1967).
Cheung M. K., Bockrath B. C.: On the specificity of UV mutagenesis inE. coli.Mutation Res. 10, 52 (1970).
Clarke C. H.: Mutation frequency decline and its relationship to excision repair.Studia Biophysica 36/37, 277 (1973).
Clarke C. H., Hill R. F.: Mutation frequency decline for streptomycine-resistant mutations induced by ultraviolet light inEscherichia coli B/r.Mutation Res. 14, 247 (1972).
Doudney C. O.: Ultraviolet light effects on the bacterial cell.Current Topics Microbiol. Immunol. 46, 46, 116 (1968).
Doudney C. O., Haas F. L.: Modification of ultra-violet induced mutation frequency and survival in bacteria by post-irradiation treatment.Proc. Natl. Acad. Sci. USA 44, 390 (1958).
Ganesan A. K., Smith K. C.: Requirement for protein synthesis inrec — dependent repair of deoxyribonucleic acid inEscherichia coli after ultraviolet or X irradiation.J. Bacteriol. 111, 575 (1972)
Green M. H. L., Rothwell M. A., Bridges B. A.: Mutation to prototrophy inEscherichia coli K-12-Effect of broth on UV-induced mutation in strain AB157 and four excision-deficient mutants.Mutation Res. 16, 225 (1972).
Haak R. A., Bockrath R. C.: Pyrimidine dimer excision and mutation frequency decline.Radiation Res. 61, 1964 (1975).
Nishioka H., Doudney C. O.: Different modes of loss of photoreversibility of ultraviolet light-induced true and supressor mutations to tryptophan independence in an auxotrophic strain ofEscherichia coli.Mutation Res. 9, 349 (1970).
Rauko P., Slameňová D.: The extent of phenotypic expression of UV-inducedstr-r andstr-d mutants inEscherichia coli B/r Hcr+ thy trp.Folia Microbiol. 19, 102 (1974).
Sedgwick S. G.: Ultraviolet inducible protein associated with error prone repair inE. coli B.Nature 255, 349 (1975).
Sedliaková M.: Influence of physiological factors on the resistance to ultraviolet radiation in bacteria.Studia Biophysica 36/37, 51 (1973).
Sedliaková M., Mašek F., Brozmanová J., Mašková E., Bugán J., Slezáriková V.: Depression of thymine dimer excision without an adequate effect on survival inEscherichia coli B/r Hor+ cells.Neoplasma,20, 499 (1973).
Skavronskaya A. G., Smirnov G. B.: UV-induction of reversions to prototrophy during growing of irradiated bacteria on enriched and deficient media. (In Russian)Genetika 5, 132 (1969).
Slameňová D.: The influence of postirradiation cultivation of the strain ofEscherichia coli B/r Hcr+ thy trp on the phenotypic expression of Trp+ mutations.Biológia 28, 949 (1973).
Turek R., Balgavý P.: The effect of a simultaneous amino acid and thymine starvation on the frequency of UV-induced Trp+ revertants in the strainEscherichia coli B/r Hcr+ thy trp. Abstract. 3rd All-Slovak Genetic Seminar, Nitra, 1973.
Věchet B.: The enhancement of the mutagenic effect of ultraviolet radiation inEscherichia coli by caffeine and criflavine.Folia Microbiol. 13, 379 (1978).
Witkin E. M.: Time, temperature and protein synthesis: A study of ultraviolet-induced mutation in bacteria.Gold Spring Harbor Symp. Quant. Biol. 21, 123 (1956).
Witkin E. M.: Ultraviolet-induced mutation and DNA repair.Ann. Rev. Genetics 3, 525 (1969).
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Balgavý, P., Rauko, P. & Turek, R. The broth effect and mutation frequency decline in cells ofEscherichia coli after irradiation with UV-light. Folia Microbiol 21, 342–354 (1976). https://doi.org/10.1007/BF02876960
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DOI: https://doi.org/10.1007/BF02876960