Abstract
The major phospholipids (PL) from soybeans and sunflowers were separated by 2-dimensional thin layer chromatography (TLC) and the fatty acid composition of each PL was determined by gas liquid chromatography (GLC). PL from soybeans and sunflowers contained high percentages of linoleic and palmitic acids. Only phosphatidylinositol (PI) from both oilseeds were similar in fatty acid composition and the principal acid was palmitic acid. Sunflower phospholipids, except for PI, contained twice as much oleic acid as did those from soybeans. Sunflower PI contained very low but measurable quantities of heptadecanoic acid. The molecular weights (MW) of individual PL were based on their fatty acid composition. The MW found for soybeans and sunflower PL were quite similar even though their fatty acid compositions were different. The average MW of PL in crude soybean and sunflower oils was determined based on the MW of individual PL and their composition in the PL fraction. From that MW, a factor for converting phosphorous content in oil to its PL content was calculated. For both oils, the factor was 25.
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Chapman, G.W. A conversion factor to determine phospholipid content in soybean and sunflower crude oils. J Am Oil Chem Soc 57, 299–302 (1980). https://doi.org/10.1007/BF02662211
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DOI: https://doi.org/10.1007/BF02662211