Summary
The effect of ATP-MgCl2 treatment was investigated on the biochemical changes of preserved kidneys and on the functional recovery of hypoxically damaged and autotransplanted canine kidneys.
We observed that ATP-MgCl2 administered before or during simple hypothermic storage did not protect the integrity of preserved kidney cells, as measured by enzyme wash-out (LDH and NAG) or by lactate release.
If the compound was administered after 120 min or 180 min clamping of the renal artery, the solitary kidney showed a faster regeneration as measured by changes in serum creatinine level. The survival rates were significantly higher in the treated groups.
Without warm ischemia of the kidney all of the autotransplanted dogs survived after surgery. After 60 min of warm ischemia the mortality rate was 100%, and the mean survival time in average 5 days. If ATP-MgCl2 was administered after the 60 min of warm ischemia, an improved recovery of the graft function was observed.
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Bergstrom J, Collste H, Groth C, Hultman E, Melin B (1979) Water electrolyte and metabolite content in cortical tissue from dog kidneys preserved by hypothermia. In: Proc EDTA. Karger, Basel New York, pp 313–321
Bore PJ, Papatheofanis I, Sells RA (1979) Adenosine triphosphate regeneration and function in the rat kidney following warm ischemia. Transplantation 27:235–237
Calman KC, Quin RO, Bell PRF (1973) Metabolic aspects of organ storage and prediction of organ viability. In Pegg G (ed) Organ preservation. Churchill-Livingstone, Edinburgh London, p 225
Chaudry IH, Sayeed MM, Baue AE (1974) Effect of hemorrhagic shock on tissue adenine nucleotides in conscious rats. Can J Physiol Pharmacol 52:131–137
Chaudry IH, Sayeed MM, Baue AE (1974) Depletion and restoration of tissue ATP in hemorrhagic shock. Arch Surg 108:208–211
Chaudry IH, Sayeed MM, Baue AE (1976) Alterations in high-energy phosphates in hemorrhagic shock as related to tissue and organ function. Surgery 79:666–668
Chaudry IH, Baue AE (1977) Depletion and replenishment of cellular cyclic adenosine monophosphate in hemorrhagic shock. Surg Gynecol Obstet 145:877–881
Collins GM, Taft P, Green RD, Ruprecht R, Halasz NA (1977) Adenine nucleotide levels in preserved and ischemically injured canine kidneys. World J Surg 1:237–243
Collins GM, Bravo-Shugarman M, Terasaki PI (1969) Kidney preservation for transportation. II. A simple technique for 12-hour storage and air transport. Transplantation 8:821–828
Fernando AR, Armstrong DMG, Griffiths JR, Hendry WF, O'Donoghue EPN, Watkinson LE (1977) Renal function after warm ischemia. II. Marked protective effect of intravenous inosine given prior to 60, 90, and 120 min of warm ischemia. Eur Urol 3:355–358
Fernando AR, Armstrong DMG, Griffiths JR, Gunter P, Hendry WF, Smith AF, Watkinson LE, Whitfield HN, Wickham JEP (1979) Prevention of renal warm ischemic injury and long-term preservation of kidneys with inosine: function, ultrastructure and clinical use. Transplant Proc 11:1477–1478
Filkins JP, Buchanan BJ (1977) Protection against endotoxin shock and impaired glucose homeostasis with ATP. Circ Shock 4:253–258
Horpacsy G, Zinsmeyer J, Schröder K, Schnells G (1977) Lysosomal and ATP changes after renal-vessel clamping in rats. Circ Shock 4:55–68
Hirasawa H, Chaudry IH, Baue AE (1978) Improved hepatic function and survival with adenosine triphosphate-magnesium chloride after hepatic ischemia. Surgery 83:655–667
Loomis ME (1961) An enzymatic fluorometric method for the determination of lactic acid in serum. J Lab Clin Med 57:966–969
Ohkawa M, Odaka M, Hirasawa H, Soeda K, Sato H (1979) Beneficial effect of ATP-MgCl2 on survival, various hepatic and reticuloendothelial function following hepatic ischemia in dogs. Eur Surg Res 11 [Suppl 1]:23
Osias MB, Siegel NJ, Chaudry IH, Lytton B, Baue AE (1977) Postischemic renal failure. Accelerated recovery with adenosine triphosphate-magnesium chloride infusion. Arch Surg 112:729–731
Price RG, Dance N, Richards R, Cattell WR (1970) The excretion of N-acetyl-β-glucosaminidase and galactosidase following surgery to the kidney. Clin Chim Acta 27:65–72
Sato F, Ito T, Akiyoshi H, Fuji S (1978) Metabolism of adenine nucleotides in the rat. J Biochem 83:1–7
Sharma G, Eiseman B (1966) Protective effects of ATP in experimental hemorrhagic shock. Surgery 59:66–74
Tarnoky K (1970) Spectrofluorometric determination of lactic-dehydrogenase in serum (Hung). Kiserl Orvostud 22:197–199
Talaat SM, Massion WH, Schilling JA (1964) Effects of adenosine triphosphate administration in irreversible hemorrhagic shock. Surgery 55:813–819
Warnick CT, Lazarus HM (1979) The maintenance of adenine nucleotide levels during kidney storage in intracellular solutions. Proc Soc Exp Biol Med 160:453–457
Zaki MS, Burke JF, Treistad RL (1978) Protective effects of adenosine triphosphate administration in burns. Arch Surg 113:605–610
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Horpàcsy, G., Gàl, G., Kiss, È. et al. Effect of ATP-MgCl2 treatment on kidney preservation and on recovery of graft function. Res. Exp. Med. 178, 3–9 (1980). https://doi.org/10.1007/BF01856752
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DOI: https://doi.org/10.1007/BF01856752