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Examination of lymphokines induced in mice following immunization with recombinant simian virus 40 large tumor antigen

  • Short Communication
  • Tumor Antigens, Tumor Immunity, Helper T-Lymphocytes, Interleukin, Interferon
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Abstract

Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was used to immunize BALB/c mice to examine the lymphokines produced following immunization. Specifically, we examined production of interleukin-2 (IL-2), IL-4, IL-5 and interferon γ (IFNγ) from immune lymphocytes cultured with decreasing concentrations of recombinant SV40 T-Ag. We identified elevated levels of IFNγ and IL-2 by enzyme-linked immunosorbent assay and a murine CTLL-2 proliferation biossay respectively. We were unable to detect either IL-4 or IL-5. These data indicate the previously reported tumor immunity induced by recombinant SV40 T-Ag immunization most likely reflects a TH1-like immune response based on thein vitro production of both IFNγ and IL-2 by immune lymphocytes.

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Authors and Affiliations

  1. Department of Virology and Immunology, Southwest Foundation for Biomedical Research, 78228, San Antonio, Texas, USA

    Robert K. Bright, Michael H. Shearer & Ronald C. Kennedy

  2. Department of Microbiology, The University of Texas Health Science Center, 78284, San Antonio, Texas, USA

    Robert K. Bright & Ronald C. Kennedy

  3. Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, College of Medicine, 73190, Oklahoma City, Oklahoma, USA

    Ronald C. Kennedy

Authors
  1. Robert K. Bright
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  2. Michael H. Shearer
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  3. Ronald C. Kennedy
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Bright, R.K., Shearer, M.H. & Kennedy, R.C. Examination of lymphokines induced in mice following immunization with recombinant simian virus 40 large tumor antigen. Cancer Immunol Immunother 40, 206–211 (1995). https://doi.org/10.1007/BF01517353

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  • DOI: https://doi.org/10.1007/BF01517353

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