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Effect of cyclic AMP and some structural analogues on the dark repair inhibition by caffeine of UV-irradiated cells

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Summary

The effect of cyclic AMP and some structural analogues on the dark-repair inhibition by caffeine has been studied. Cyclic AMP had no significant effect, contrary to the previous report by Wackeret al. [5], on the dark-repair inhibition by caffeine. Some cyclic AMP derivatives, resistent to hydrolysis by the phosphodiesterase enzyme, were used to investigate the role of phosphodiesterase in dark-repair inhibition by caffeine. These derivatives, when added alone, had no activity on the dark-repair process. Experiments in combination with caffeine showed that these derivatives were unable to reverse the dark-repair inhibition by caffeine. The present data do not support any correlation between the caffeine inhibition of the dark-repair process, and the phosphodiesterase involved in cyclic AMP metabolism.

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Authors and Affiliations

  1. Gustav Embden-Zentrum der Biologischen Chemie, Universität Frankfurt (Main), Federal Republic of Germany

    P. Chandra, R. E. O. Scheckel & A. Wacker

  2. Abt. für Molekularbiologie, Gustav Embden-Zentrum der Biologischen Chemie, Theodor Stern-Kai 7, D-600, Frankfurt (Main) 70, Federal Republic of Germany

    P. Chandra

Authors
  1. P. Chandra
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  2. R. E. O. Scheckel
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  3. A. Wacker
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Chandra, P., Scheckel, R.E.O. & Wacker, A. Effect of cyclic AMP and some structural analogues on the dark repair inhibition by caffeine of UV-irradiated cells. Radiat Environ Biophys 11, 87–90 (1974). https://doi.org/10.1007/BF01323104

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  • DOI: https://doi.org/10.1007/BF01323104

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