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A comparative evaluation of methods for determining the activity of chymotrypsin

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Summary

A method has been developed for determining the activity of chymotrypsin with respect to the coagulation of milk-acetate mixture in N. P. Pyatnitskii's modification. In specificity and sensitivity, this method approaches that based on the hydrolysis of N-acetyl-D, L-phenylalanine p-nitroanilide.

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References

  1. P. G. Storozhuk, Voprosy kurortologii, fizioterapii i lech. fizkul'tury, no. 6, 498–503, 1961.

  2. N. P. Pyatnitskii and B. S. Mikhalenko, Izv. vyssh. uch. zaved., Pishchevaya tekhnol., no. 4, 147–149, 1965.

  3. J. Tang, S. Wolf, R. Caputto, and R. Trucco, J. Biol. Chem., 234, no. 5, 1174–1178, 1959.

    CAS  PubMed  Google Scholar 

  4. M. Seijffers, H. Segal, and L. Miller, Amer. J. Physiol., 207, no. 1, 8–12, 1964.

    CAS  PubMed  Google Scholar 

  5. H. Tuppy, U. Wiesbauer, and E. Wintersberger, Hoppe Seyl. Z., 329, no. 3–6, 278–288, 1962.

    Article  CAS  Google Scholar 

  6. R. A. Matveeva, Ya. I. Lapuk, and V. M. Stepanov, Izv. AN SSSR, ser. khim., no. 3, 213, 1964.

  7. J. H. Northrop, M. Kunitz, and R. M. Herriott, Crystalline Enzymes, 2nd ed., 262, Columbia University Press, New York, 1948.

    Google Scholar 

  8. N. P. Pyatnitskii and M. N. Selyukova, Voprosy pitaniya, no. 2, 176, 1960.

  9. N. G. Belen'kii and L. B. Polonskaya, Alpha-Chymotrypsin [in Russian], 48, Moscow, 1962.

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Khimiya Prirodnykh Soedinenii, Vol. 4, No. 1, pp. 32–34, 1968

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Proskuryakov, M.T. A comparative evaluation of methods for determining the activity of chymotrypsin. Chem Nat Compd 4, 25–26 (1968). https://doi.org/10.1007/BF00567757

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  • DOI: https://doi.org/10.1007/BF00567757

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