Skip to main content
SpringerLink
Log in

Characterization of a human genomic DNA fragment coding for a myosin heavy chain

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A DNA segment from the human genome with information for myosin heavy chain (MHC) was isolated from a human genomic DNA library cloned in λ Charon 4A phages. The isolation was accomplished by a myosin cDNA probe obtained from rabbit heart muscle mRNA (Sinha et al. 1982). The selected human DNA clone, designated λ gMHCl, contains a genomic DNA fragment of about 14 kilobase pairs. The transcriptional polarity of this DNA was determined. The 5′-end of the gene is missing from the cloned fragment. This human gene exhibits sequence homology to MHC DNA of rabbit and chicken, but not to an MHC sequence of nematode. The isolated gene fragment is a member of the human MHC multigene family, which is presumed to consist of probably more than ten separate sarcomeric MHC genes per haploid genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benton WD, Davis RW (1977) Screening λ gt recombinant clones by hybridization to single plaques in situ. Science 196:180–182

    Google Scholar 

  • Bernstein SI, Mogami K, Donady JJ, Emerson CP Jr (1983) Drosophila muscle myosin heavy chain encoded by a single gene in a cluster of muscle mutations. Nature 302:393–397

    Google Scholar 

  • Blattner FR, Williams BG, Blechl AE, Denniston-Thompson K, Faber HE, Furlong L-A, Grunwald DJ, Kiefer DO, Moore DD, Schumm JW, Sheldon EL, Smithies O (1977) Charon phages: safer derivatives of bacteriophage lambda for DNA cloning. Science 196:161–169

    Google Scholar 

  • Chizzonite RA, Everett AW, Clark WA, Jakovcic S, Rabinowitz M, Zak R (1982) Isolation and characterization of two molecular variants of myosin heavy chain from rabbit ventricle. Change in their content during normal growth and after treatment with thyroid hormone. J Biol Chem 257:2056–2065

    Google Scholar 

  • Clarke L, Carbon J (1976) A colony bank containing synthetic ColE1 hybrid plasmids representative of the entire E. coli genome. Cell 9:91–99

    Google Scholar 

  • Cummins P (1983) Contractile proteins in muscle disease. J Muscle Res Cell Motil 4:5–24

    Google Scholar 

  • Elzinga M, Trus B (1980) Sequence and proposed structure of a 17,000 dalton fragment of myosin. In: Birr C (ed) Methods in peptide and protein sequence analysis. Elsevier/North-Holland, Amsterdam New York Oxford, pp 213–224

    Google Scholar 

  • Engel J, Gunning P, Kedes L (1982) Human actin proteins are encoded by a multigene family. In: Pearson ML, Epstein HF (eds) Muscle development, molecular and cellular control Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 107–117

    Google Scholar 

  • Everett AW, Clark WA, Chizzonite RA, Zak R (1983) Change in synthesis rates of α- and β-myosin heavy chain in rabbit heart after treatment with thyroid hormone. J Biol Chem 258:2421–2425

    Google Scholar 

  • Flink IL, Rader JH, Morkin E (1979) Thyroid hormone stimulates synthesis of a cardiac myosin isozyme. Comparison of the twodimensional electrophoretic patterns of the cyonogen bromide peptides of cardiac myosin heavy chains from euthyroid and thyrotoxic rabbits. J Biol Chem 254:3105–3110

    Google Scholar 

  • Gauthier GF, Lowey S (1979) Distribution of myosin isoenzymes among skeletal muscle fiber types. J Cell Biol 81:10–25

    Google Scholar 

  • Gauthier GF, Lowey S, Hobbs AW (1978) Fast and slow myosin in developing muscle fibers. Nature 274:25–29

    Google Scholar 

  • Hoh JFY (1975) Neural regulation of mammalian fast and slow muscle myosin: An electrophoretic analysis. Biochemistry 14: 742–747

    Google Scholar 

  • Hoh JFY, Yeoh GPS (1980) Rabbit skeletal myosin isoenzymes from fetal, fast-twitch and slow-twitch muscles. Nature 280: 321–323

    Google Scholar 

  • Hoh JFY, McGrath PA, Hale PT (1977) Electrophoretic analysis of multiple forms of rat cardiac myosin: effects of hypophysectomy and thyroxine replacement. J Mol Cell Cardiol 10:1053–1076

    Google Scholar 

  • Hoh JFY, Yeoh GPS, Thomas MAW, Higginbottom L (1979) Structural differences in the heavy chains of rat ventricular myosin isoenzymes. FEBS Lett 97:330–334

    Google Scholar 

  • Huszar G (1972) Developmental changes of the primary structure and histidine methylation in rabbit skeletal muscle myosin. Nature New Biol 240:260–264

    Google Scholar 

  • Jakowlew SB, Khandekar P, Datta K, Arnold H-H, Narula SK, Siddiqui MAQ (1982) Molecular cloning and expression of cardiac-specific myosin heavy chain gene sequences in chick embryo. J Mol Biol 156:673–682

    Google Scholar 

  • Karn J, Brenner S, Barnett L (1983) Protein structural domains in the Caenorhabditis elegans unc-54 myosin heavy chain gene are not separated by introns. Proc Natl Acad Sci USA 80:4253–4257

    Google Scholar 

  • Kavinsky CJ, Umeda PK, Sinha AM, Elzinga M, Tong SW, Zak R, Jakovcic S, Rabinowitz M (1983) Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. I. DNA and derived amino acid sequence of light meromyosin. J Biol Chem 258:5196–5205

    Google Scholar 

  • Lawn RM, Fritsch EF, Parker RC, Blake G, Maniatis T (1978) The isolation and characterization of linked α- and β-globin genes from a cloned library of human DNA. Cell 15:1157–1174

    Google Scholar 

  • Leder P, Tiemeier D, Enquist L (1977) EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λ gtWES system. Science 196:175–177

    Google Scholar 

  • Leinwand LA, Saez L, McNally E, Nadal-Ginard B (1983) Isolation and characterization of human myosin heavy chain genes. Proc Natl Acad Sci USA 80:3716–3720

    Google Scholar 

  • Lowey S, Benfield PA, LeBlanc DD, Waller G, Winkelmann DA, Gauthier GF (1982) Characterization of myosin from embryonic and developing chicken pectoralis muscle. In: Pearson ML, Epstein HF (eds) Muscle development, molecular and cellular control. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 15–24

    Google Scholar 

  • MacLeod AR, Karn J, Brenner S (1981) Molecular analysis of the unc-54 myosin heavy chain gene of Caenorhabditis elegans. Nature 291:386–390

    Google Scholar 

  • Mahdavi V, Periasamy M, Nadal-Ginard B (1982) Molecular characterization of two myosin heavy chain genes expressed in the adult heart. Nature 297:659–664

    Google Scholar 

  • Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Masaki T, Yoshizaki C (1974) Differentiation of myosin in chick embryos. J Biochem (Tokyo) 76:123–131

    Google Scholar 

  • McLachlan AD, Karn J (1982) Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle. Nature 299:226–231

    Google Scholar 

  • Nguyen HT, Gubits RM, Wydro RM, Nadal-Ginard B (1982) Sarcomeric myosin heavy chain is coded by a highly conserved multigene family. Proc Natl Acad Sci USA 79:5230–5234

    Google Scholar 

  • Rozek CE, Davidson N (1983) Drosophila has one myosin heavy-chain gene with three developmentally regulated transcripts. Cell 32:23–34

    Google Scholar 

  • Rubinstein NA, Holtzer H (1979) Fast and slow muscles in tissue culture synthesise only fast myosin. Nature 280:323–325

    Google Scholar 

  • Rushbrook JI, Stracher A (1979) Comparison of adult, embryonic, and dystrophic myosin heavy chains from chicken muscle by sodium dedecylsulfate/polyacrylamide gel electrophoresis and peptide mapping. Proc Natl Acad Sci USA 76:4331–4334

    Google Scholar 

  • Sinha AM, Umeda PK, Kavinsky CJ, Rajamanickam C, Hsu H-J, Jakovcic S, Rabinowitz M (1982) Molecular cloning of mRNA sequences for cardiac- and β-form heavy chains: expression in ventricles of normal, hypothyroid, and thyrotoxic rabbits. Proc Natl Acad Sci USA 79:5847–5851

    Google Scholar 

  • Souther EM (1975) detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  • Umeda PK, Zak R, Rabinowitz M (1980) Purification of ribonucleic acids for fast and slow myosin heavy chains by indirect immunoprecipitation of polysomes from embryonic chick skeletal muscle. Biochemistry 19:1955–1965

    Google Scholar 

  • Umeda PK, Sinha AM, Jakovcic S, Merten S, Hsu H-J, Subramanian KN, Zak R, Rabinowitz M (1981) Molecular cloning of two fast myosin heavy chain cDNAs from chicken embryo skeletal muscle. Proc Natl Acad Sci USA 78:2843–2847

    Google Scholar 

  • Umeda PK, Kavinsky CJ, Sinha AM, Hsu H-J, Jakovcic S, Rabinowitz M (1982) Differential expression of two fast myosin heavy-chain mRNAs during chicken skeletal-muscle development. In: Pearson ML, Epstein HF (eds) Muscle development, molecular and cellular control. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 169–176

    Google Scholar 

  • Umeda PK, Kavinsky CJ, Sinha AM, Hsu H-J, Jakovcic S, Rabinowitz M (1983) Cloned mRNA sequences for two types of embryonic heavy chains from chick skeletal muscle. II. Expression during development using S1 nuclease mapping. J Biol Chem 258:5206–5214

    Google Scholar 

  • Whalen RG, Sell SM, Butler-Browne GS, Schwartz K, Bouveret P, Pinset-Härström I (1981) Three myosin heavy-chain isozymes appear sequentially in rat muscle development. Nature 292: 805–809

    Google Scholar 

  • Wydro RM, Nguyen HT, Gubits RM, Nadal-Ginard B (1983) Characterization of sarcomeric myosin heavy chain genes. J Biol Chem 258:670–678

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung Molekulare Biologie, Max-Planck-Institut für medizinische Forschung, Jahnstr. 29, D-6900, Heidelberg, Germany

    H. Appelhans & H.-P. Vosberg

Authors
  1. H. Appelhans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H.-P. Vosberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Appelhans, H., Vosberg, HP. Characterization of a human genomic DNA fragment coding for a myosin heavy chain. Hum Genet 65, 198–203 (1983). https://doi.org/10.1007/BF00286664

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00286664

Keywords

Search

Navigation