Abstract
Use of leaf meters to provide an instantaneous assessment of leaf chlorophyll has become common, but calibration of meter output into direct units of leaf chlorophyll concentration has been difficult and an understanding of the relationship between these two parameters has remained elusive. We examined the correlation of soybean (Glycine max) and maize (Zea mays L.) leaf chlorophyll concentration, as measured by organic extraction and spectrophotometric analysis, with output (M) of the Minolta SPAD-502 leaf chlorophyll meter. The relationship is non-linear and can be described by the equation chlorophyll (μmol m−2)=10(M0.265), r 2=0.94. Use of such an exponential equation is theoretically justified and forces a more appropriate fit to a limited data set than polynomial equations. The exact relationship will vary from meter to meter, but will be similar and can be readily determined by empirical methods. The ability to rapidly determine leaf chlorophyll concentrations by use of the calibration method reported herein should be useful in studies on photosynthesis and crop physiology.
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Abbreviations
- Chl-:
-
chlorophyll
- M-:
-
SPAD-502 meter value
References
Arnon DI (1949) Copper enzymes in isolated chloroplasts: Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24: 1–15
Blackmer TM, Schepers JS and Vigil MF (1993) Chlorophyll meter readings in corn as affected by plant spacing. Commun Soil Sci Plant Anal 24: 2507–2516
Fox RH, Piekielek WP and Macneal KM (1994) Using a chlorophyll meter to predict nitrogen fertilizer needs of winter wheat. Commun Soil Sci Plant Anal 25: 171–181
Fukshansky L, Martinez v. Remisowsky A, McClendon J, Ritterbosch A, Richter T and Mohr H (1993) Absorption spectra of leaves corrected for scattering and distributional error: A radiative transfer and absorption statistics treatment. Photochem Photobiol 57: 538–555
Hardwick K and Baker NR (1973) In vivo measurement of chlorophyll content of leaves. New Phytol 72: 51–54
Holden M (1976) Chlorophylls. In: Goodwin TW (ed) Chemistry and Biochemistry of Plant Pigments, pp 1–37. Academic Press, New York
Inada K (1965) Studies on a method for determining the deepness of green color and chlorophyll content of intact crop leaves and its practical applications. 2. Photoelectric characters of chlorophyllo-meter and correlation between the reading and chlorophyll content in leaves. Proc Crop Sci Soc Japan 33: 301–308
Kaakeh W, Pfeiffer DG and Marini RP (1992) Combined effects of spirea aphid (Homoptera: Aphididae) and nitrogen fertilization on net photosynthesis, total chlorophyll content, and greenness of apple leaves. J Econ Entomol 85: 939–946
Kirk JTO (1994) Light and Photosynthesis in Aquatic Ecosystems. Cambridge University Press, Cambridge
MacNicol PK, Dudzinski ML and Condon BN (1976) Estimation of chlorophyll in tobacco leaves by direct photometry. Ann Bot 40: 143–152
McClendon JH and Fukshansky L (1990a) On the interpretation of absorption spectra of leaves. II. The non-absorbedray of the sieve effect and the mean optical pathlength in the remainder of the leaf. Photochem Photobiol 51: 211–216
McClendon JH and Fukshansky L (1990b) On the interpretation of absorption spectra of leaves. I. Introduction and the correction of leaf spectra for surface reflection. Photochem Photobiol 51: 203–210
McClure WF (1969) Fiber-optic spectrophotometer for in vivo analysis of biological materials: Chlorophyll measurements. Trans Am Soc Agric Eng 12: 319–321
Monje OA and Bugbee B (1992) Inherent limitations of nondestructive chlorophyll meters: A comparison of two types of meters. HortScience 27: 69–71
Myers DA, Vogelmann TC and Bornman JF (1994) Epidermal focusing and effects on light utilization in Oxalis acetoselld. Physiol Plant 91: 651–656
Nobel PS (1991) Physiochemical and Environmental Plant Physiology. Academic Press, New York
Piekielek WP and Fox RH (1992) Use of a chlorophyll meter to predict sidedress nitrogen requirements for maize. Agron J 84: 59–65
Porra RJ, Thompson WA and Kreidemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975: 384–394
Rabinowitch EI (1951) Photosynthesis and Related Processes. Interscience Publishers, Inc., New York
Turner FT and Jund MF (1991) Chlorophyll meter to predict nitrogen topdress requirement for semidwarf rice. Agron J 83: 926–928
Uz M and Saygin O (1994) Determination of the light pathlength elongation in leaves by measuring P700 quantitatively. Photosynth Res 40: 175–179
Vogelmann TC (1993) Plant tissue optics. Annu Rev Plant Physiol Plant Mol Biol 44: 231–251
Wood CW, Reeves DW, Duffield RR and Edmisten KL (1992a) Field chlorophyll measurements for evaluation of corn nitrogen status. J Plant Nutri 15: 487–500
Wood CW, Tracy PW, Reeves DW and Edmisten KL (1992b) Determination of cotton nitrogen status with a hand-held chlorophyll meter. J Plant Nutri 15: 1435–1448
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Markwell, J., Osterman, J.C. & Mitchell, J.L. Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynth Res 46, 467–472 (1995). https://doi.org/10.1007/BF00032301
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DOI: https://doi.org/10.1007/BF00032301