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Calpain Activity and Expression in Human Colonic Tumors

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Pathophysiological Aspects of Proteases

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Abstract

Families of cysteine proteases which are activated by Ca2+ are known as calpains. These enzymes can contribute to the metastatic behavior of certain tumors. In particular, we have found from Western Blotting and immunohistochemistry that the activity and expression of m-calpain is higher in colorectal tumors than non-malignant colonic mucosa. Furthermore, the expression of calpastin and high molecular weight calmodulin-binding protein, which are inhibitors of calpains, is reduced in colon cancers. These findings are of great importance in the design and development of anticancer drugs as well as providing diagnostic value as an indicator of the development of colorectal cancer. This chapter is devoted principally to the discoveries in our laboratories of calpain and its inhibitors in relation to human colonic neoplasms.

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Acknowledgements

This study was supported by the Canadian Institutes of Health Research, Canada.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada

    Rajendra K. Sharma, Sreejit Parameswaran & Sujeet Kumar

  2. College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, S7N 5C9, Canada

    Umashankar Das & Jonathan R. Dimmock

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  1. Rajendra K. Sharma
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  2. Umashankar Das
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  3. Sreejit Parameswaran
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Corresponding author

Correspondence to Rajendra K. Sharma .

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Editors and Affiliations

  1. Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India

    Sajal Chakraborti

  2. Institute of Cardiovascular Sciences, University of Manitoba, Manitoba, Canada

    Naranjan S. Dhalla

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Sharma, R.K., Das, U., Parameswaran, S., Kumar, S., Dimmock, J.R. (2017). Calpain Activity and Expression in Human Colonic Tumors. In: Chakraborti, S., Dhalla, N. (eds) Pathophysiological Aspects of Proteases. Springer, Singapore. https://doi.org/10.1007/978-981-10-6141-7_7

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