Abstract
Cisplatin (CDDP) is a widely used platinum-based compound that is effective against a broad spectrum of solid neoplasms. Although CDDP often leads to an initial therapeutic success, associated with partial responses or disease stabilization, many patients are intrinsically resistant to cisplatin-based therapies. The essential cytotoxic factors of photodynamic treatment (PDT) are reactive oxygen species (ROS). The reaction of ROS with cytoplasmic peptides and proteins may reduce the degree of inactivation of CDDP. In this study, we applied low-level PDT, which demonstrated no remarkable cytotoxic effects on CDDP–resistant HeLa cells (HeLa/CDDP) and examined whether PDT can reduce the CDDP-resistance level. There were no remarkable cytotoxic effects without CDDP in the PDT. The viability of the HeLa/CDDP-PDT group at a CDDP concentration of 10 µM was equal to that of normal HeLa cells. These findings suggest that PDT, which does not have sufficient cytotoxic effects, reduces the resistance level of HeLa/CDDP.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Naito, S., Yokomizo, A., Koga, H.: Mechanisms of drug resistance in chemotherapy for urogenital carcinoma. Int J. Urol. 6, 427–439 (1999)
Galluzzi, L., Senovilla, L., Vitale, I., Michels, I., Kepp, O., Castedo, M., Kroemer, G.: Molecular mechanisms of cisplatin resistance. Oncogene 31(15), 1869-1883 (2012)
Dougherty, T. J.: Photodynamic therapy. Photochem. Photobiol. 58, 895-900 (1993)
Miyamoto, Y., Nishikiori, D., Hagino, F., Wakita, M., Tanabe, I., Toida, M.: Effect of 630-nm pulsed laser irradiation on the proliferation of HeLa cells in Photofrin-mediated photodynamic therapy. Laser Therapy 20(2), 135-138 (2011)
Nishikiori, D., Miyamoto, Y.: Enhancement of the cytotoxic effects of bleomycin with permeabilization of the plasma membrane by Photofrin-mediated photodynamic therapy in vitro. IFMBE Proceedings 43, 711-713 (2013)
Takara, K., Obata, Y., Yoshikawa, E., Kitada, N., Sakaeda, T., Ohnishi, N., Yokoyama, T.: Molecular changes to HeLa cells on continuous exposure to cisplatin or paclitaxel. Cancer Chemother Pharmacol. 58, 785-793 (2006)
Bi, W., Wang, Y., Sun, G., Zhang, X., Wei, Y., Li, L., Wang, X.: Paclitaxel-resistant HeLa cells have up-regulated levels of reactive oxygen species and increased expression of taxol resistance gene 1. Pak J Pharm Sci. 27(4), 871-878 (2014)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Miyamoto, Y., Yamaguchi, Y., Tajima, H. (2017). Effects of Photofrin-mediated photodynamic treatment on sensitivity to cisplatin in HeLa cells and the resistant subline. In: Goh, J., Lim, C., Leo, H. (eds) The 16th International Conference on Biomedical Engineering. IFMBE Proceedings, vol 61. Springer, Singapore. https://doi.org/10.1007/978-981-10-4220-1_3
Download citation
DOI: https://doi.org/10.1007/978-981-10-4220-1_3
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-4219-5
Online ISBN: 978-981-10-4220-1
eBook Packages: EngineeringEngineering (R0)