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Somatic Embryogenesis in Loblolly Pine (Pinus Taeda L.)

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Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 44-46))

Abstract

Somatic embryogenesis, the formation and development of embryos from somatic (vegetative) tissues under in vitro conditions, was first discovered in carrot (Steward et al., 1958) and in conifers with Picea abies in 1985 (Hakman et al., 1985). The first report of somatic embryogenesis with Pinus taeda was in 1987 (Gupta & Durzan, 1987).

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References

  • Attree, S.M., M.K. Pomeroy & L.C. Fowke, 1992. Manipulation of conditions for the culture of somatic embryos of white spruce for improved triacylglycerol biosynthesis and desiccation tolerance. Planta 187: 395–404.

    Article  CAS  Google Scholar 

  • Balocchi, C.E., F.E. Bridgewater & R. Bryant, 1994. Selection efficiency in a non-selected population of loblolly pine. For. Sci., in press.

    Google Scholar 

  • Becwar, M.R., S.R. Wann, M.A. Johnson, S.A. Verhagen, R.P. Feirer & R. Nagmani, 1988. Development and characterization of in vitro embryogenic systems in conifers. In: M.R. Ahuja (Ed). Somatic cell genetics of woody plants, pp. 1–18. Kluwer Academic Publishers, Dordrecht.

    Chapter  Google Scholar 

  • Becwar, M.R., T.D. Blush, D.W., Brown & E.E. Chesick, 1991. Multiple paternal genotypes in embryogenic tissue derived from individual immature loblolly pine seeds. Plant Cell Tiss. Org. Cult. 26: 37–44.

    Article  Google Scholar 

  • Becwar, M.R., R. Nagmani & S.R. Wann, 1990. Initiation of embryogenic cultures and somatic embryo development in loblolly pine (Pinus taeda). Can. J. For. Res. 20: 810–817.

    Article  Google Scholar 

  • Boyer, J.N. & D.B. South, 1984. Forest nursery practices in the south. Southern J. Applied For. 8: 67–75.

    Google Scholar 

  • Buchholz, J.T., 1920. Embryo development and polyembryony in relation to the phylogeny of conifers. Amer. J. Bot. 7: 125–145.

    Article  Google Scholar 

  • Chandler, S.F., C. Bateman, C. Blomstedt, D. Willyams & R. Young, 1989. Forestry biotechnology at Calgene Pacific. Aust. J. Biotech. 3: 281–284.

    Google Scholar 

  • Eastman, P.A.K., F.B. Webster, J.A. Pitel & D.R. Roberts, 1991. Evaluation of somaclonal variation during somatic embryogenesis of interior spruce (Picea glauca engelmannii complex) using culture morphology and isozyme analysis. Plant Cell Rep. 10: 425–430.

    Article  Google Scholar 

  • Finer, J.J., H. Kriebel & M.R. Becwar, 1989. Initiation of embryogenic callus and suspension cultures of white pine (Pinus strobus L.). Plant Cell Rep. 8: 203–206.

    Article  Google Scholar 

  • Gates, J.C. & M.S. Greenwood, 1991. The physical and chemical environment of the developing embryo of Pinus resinosa. Amer. J. Bot. 8: 1002–1009.

    Article  Google Scholar 

  • Gupta, P.K. & D.J. Durzan, 1986. Somatic polyembryogenesis from callus of mature sugar pine embryos. Bio/Technology 4: 643–645.

    Article  Google Scholar 

  • Gupta, P.K. & D.J. Durzan, 1987. Biotechnology of somatic polyembryogenesis and plantlet regeneration in loblolly pine. Bio/Technology 5: 147–151.

    Article  Google Scholar 

  • Gupta, P.K. & D.J. Durzan, 1991. Loblolly pine (Pinus taeda L.). In: Y.P.S. Bajaj (Ed). Biotechnology in agriculture and forestry, vol. 16, Trees III, pp. 383–407, Springer-Verlag, Berlin.

    Google Scholar 

  • Gupta, P.K. & G.S. Pullman, 1990. Method for reproducing coniferous plants by somatic embryogenesis. U.S. Patent No. 4,957,866.

    Google Scholar 

  • Gupta, P.K. & G.S. Pullman, 1991. Method for reproducing coniferous plants by somatic embryogenesis using abscisic acid and osmotic potential variation. U.S. Patent No. 5,036,007.

    Google Scholar 

  • Gupta, P.K. & G.S. Pullman, 1993. Method for reproducing conifers by somatic embryogenesis using stepwise hormone adjustment. U.S. Patent No. 5,236,841.

    Google Scholar 

  • Gupta, P.K., D.J. Durzan & B.J. Finkle, 1987. Somatic polyembryogenesis in embryogenic cell masses of Picea abies (Norway spruce) and Pinus taeda (loblolly pine) after thawing from liquid nitrogen. Can. J. For. Res. 17: 1130–1133.

    Article  Google Scholar 

  • Gupta, P.K., G. Pullman, R. Timmis, M. Kreitinger, W. Carlson, J. Grob & E. Welty, 1993. Forestry in the 21st century: The biotechnology of somatic embryogenesis. Bio/Technology 11: 454–459.

    Article  Google Scholar 

  • Hakman, I. & L.C. Fowke, 1987. Somatic embryogenesis in Picea glauca (white spruce) and P. mariana (black spruce). Can. J. Bot. 65: 656–659.

    Article  Google Scholar 

  • Hakman, I., L.C. Fowke, S. von Arnold & T. Eriksson, 1985. The development of somatic embryos in tissue cultures initiated from immature embryos of Picea abies (Norway spruce). Plant Sci. 38: 53–59.

    Article  Google Scholar 

  • Isabel, N., L. Tremblay, M. Michaud, F.M. Tremblay & J. Bousquet, 1993. RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. Theor. Appl. Genet. 86: 81–87.

    Article  CAS  Google Scholar 

  • Jain, S.M., N. Dong & R.J. Newton, 1989. Somatic embryogenesis in slash pine (Pinus elliottii) from immature embryos cultured in vitro. Plant Sci. 65: 233–241.

    Article  Google Scholar 

  • Jones, N.B., J. van Staden, A.D. Bayley, 1993. Somatic embryogenesis in Pinus patula. J. Plant Physiol. 142: 366–372.

    Article  CAS  Google Scholar 

  • Kapik, R., R. Dinus & J.F.D. Dean, 1993. Abscisic acid during zygotic embryogenesis in Pinus taeda L. (Abstract). In: Biology and control of reproductive processes in forest trees, IUFRO Symposium Section S2.01–05, Aug 22–26, 1993. Victoria, British Columbia, Canada.

    Google Scholar 

  • Kapik, R., R. Dinus & J.F.D. Dean, 1994. Abscisic acid during zygotic embryogenesis in Pinus taeda L. Tree Physiol., in review.

    Google Scholar 

  • Klimaszewska, K., C. Ward & W.M. Cheliak, 1992. Cryopreservation and plant regeneration from embryogenic cultures of larch (Larix x eurolepis) and black spruce (Picea mariana). J. Exp. Bot. 43: 73–79.

    Article  CAS  Google Scholar 

  • Lackie, J.M. & J.A.T. Dow, 1989. The dictionary of cell biology. Academic Press, London.

    Google Scholar 

  • Laine, E. & A. David, 1990. Somatic embryogenesis in immature embryos and protoplasts of Pinus caribaea. Plant Sci. 69: 215–224.

    Article  Google Scholar 

  • Laine, E., P. Bade & A. David, 1992. Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea. Plant Cell Rep. 11: 295–298.

    Article  Google Scholar 

  • Little, E.L., 1971. Atlas of United States trees. U.S. Dept. Agric, Forest Service Misc. Publ. No. 1146, Washington, D.C.

    Google Scholar 

  • Moulton, R.J., R.M. Mangold & J.D. Snellgrove, 1993. Tree planting in the United States 1992. USDA Forest Service Publ., Washington, D.C.

    Google Scholar 

  • Nagmani R., A.M. Diner & G.C. Sharma, 1993. Somatic embryogenesis in longleaf pine (Pinus palustris). Can. J. For. Res. 23: 873–876.

    Article  CAS  Google Scholar 

  • Pullman, G.S. & P.K. Gupta, 1991. Method for reproducing coniferous plants by somatic embryogenesis using absorbent materials in the development stage media. U.S. Patent No. 5,034,326.

    Google Scholar 

  • Schaeffer, W., 1990. Terminology associated with cell, tissue and organ culture, molecular biology and molecular genetics. In Vitro Cell. Dev. Biol. 26: 97–101.

    Article  PubMed  CAS  Google Scholar 

  • Smith, D.R., A.P. Singh & L. Wilton, 1985. Zygotic embryos of Pinus radiata in vivo and in vitro. In: Smith, D.R. (Ed). Abstracts, International Conifer Tissue Culture Working Group, 12–16 Aug 1985, p. 21. Forest Research Institute, New Zealand Forest Service, Rotorua, New Zealand.

    Google Scholar 

  • Steward, F.C., M.O. Mapes & K. Mears, 1958. Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Amer. J. Bot. 45: 705–708.

    Article  Google Scholar 

  • Tautorus, T.E., L.C. Fowke & D.I. Dunstan, 1991. Somatic embryogenesis in conifers. Can. J. Bot. 69: 1873–1899.

    Article  Google Scholar 

  • Uddin, M., 1993. Somatic embryogenesis in gymnosperms. U.S. Patent No. 5, 187, 092.

    Google Scholar 

  • Uddin, M.R., R.J. Dinus & D.T. Webb, 1990. Effects of different carbohydrates on maturation of Pinus taeda somatic embryos. Abstracts VII International Congress on Plant Tissue and Cell Culture, June 24–29, 1990, p. 272. Amsterdam, Netherlands.

    Google Scholar 

  • Walker, L.C., 1990. Forests: A naturalist’s guide to trees and forest ecology. John Wiley & Sons, Inc., N.Y.

    Google Scholar 

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Authors
  1. Michael R. Becwar
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  2. Gerald S. Pullman
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Editor information

Editors and Affiliations

  1. Department of Plant Production, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Weyerhaeuser Inc., Tacoma, Washington, USA

    Pramod K. Gupta

  3. Department of Forest Science, Texas A & M University, College Station, Texas, USA

    Ronald J. Newton

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© 1995 Springer Science+Business Media Dordrecht

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Becwar, M.R., Pullman, G.S. (1995). Somatic Embryogenesis in Loblolly Pine (Pinus Taeda L.). In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 44-46. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0960-4_18

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  • DOI: https://doi.org/10.1007/978-94-011-0960-4_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4415-8

  • Online ISBN: 978-94-011-0960-4

  • eBook Packages: Springer Book Archive

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