Abstract
Molecular genetics has rapidly become an integral tool for elucidating the molecular mechanisms of photosynthesis. In the bacterial reaction center (RC) protein, structure/function questions, raised through an inspection of the X-ray crystallographic structure, have been addressed by the combined approaches of genetics and spectroscopy. This system is somewhat unique in that relatively simple, yet powerful, spectroscopic techniques can be used to evaluate the structure and function of these genetically altered proteins. Using recombinant DNA technology, mutations have now been constructed in the vicinity of all nine prosthetic groups in the reaction center from Rb. capsulatus. Mutations have also been directed at the bacteriochlorophyll binding sites of the associated light harvesting antennae. The spectroscopic characterization of genetically-altered RCs has been recently reviewed (1).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Coleman, W.J. and Youvan, D.C. 1990. Genetic and Biophysical Analyses of the Light Reactions of Photosynthesis. Annual Review of Biophysics and Biophysical Chemistry, submitted.
Youvan, D.C., Ismail, S., and Bylina, E.J. 1985. Chromosomal Deletion and Plasmid Complementation of the Photosynthetic Reaction Center and Light-Harvesting Genes from Rhodopseudomonas capsulata. Gene 38:19–30.
Bylina, E.J., Jovine, R.V.M., and Youvan, D.C. 1989. A Genetic System for Rapidly Assessing Herbicides That Compete for the Quinone Binding Site of Photosynthetic Reaction Centers. Bio/Technologv 7:69–74.
Bylina, E.J., and Youvan, D.C. 1987. Genetic Engineering of Herbicide Resistance: Saturation Mutagenesis of IL229 of the Reaction Center L Subunit. Z. Naturforsch. 42c:769–774.
Bylina, E.J., Kirmaier, C., McDowell, L., Holten, D., and Youvan, D.C. 1988. Influence of an Amino Acid Residue on the Optical Properties and Electron Transfer Dynamics of a Photosynthetic Reaction Centre Complex. Nalure, 336:182–184.
Bylina, E.J. and Youvan, D.C. 1988. Directed Mutations Affecting Spectroscopic and Electron Transfer Properties of the Primary Donor in the Photosynthetic Reaction Center. PNAS, 85:7226–7230.
Kirmaier, C., Holten, D., Bylina, E.J., and Youvan, D.C. 1988. Electron Transfer in a Genetically Modified Reaction Center Containing a Heterodimer. PNAS, 85:7562–7566.
Bylina, E.J., Kolaczkowski, S.V., Norris, J.R., and Youvan, D.C. 1990. EPR Characterization of Genetically Modified Reaction Centers of Rhodobacter capsulatus, submitted.
Breton, J., Bylina, E.J. and Youvan, D.C. 1989. Pigment Organization in Genetically Modified Reaction Centers of Rhodobacter capsulatus. Biochemistry, in press.
Prince, R.C. and Youvan, D.C. 1987. Isolation and Spectroscopic Properties of Photochemical Reaction Centers from Rhodobacter capsulatus. BBA, 890: 286–291.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media New York
About this chapter
Cite this chapter
Bylina, E.J., Youvan, D.C. (1990). Mutagenesis of Reaction Center Histidine L173 Yields an L-Side Heterodimer. In: Baltscheffsky, M. (eds) Current Research in Photosynthesis. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0511-5_8
Download citation
DOI: https://doi.org/10.1007/978-94-009-0511-5_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6716-4
Online ISBN: 978-94-009-0511-5
eBook Packages: Springer Book Archive