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Liquid Extraction: Phase Diagram

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Encyclopedia of Lipidomics

Liquid Extraction: Phase Diagram

Liquid extraction of lipids from biological samples has greater efficiencies through their better solubility of hydrocarbon chains in organic solvents. One of the most common extraction methods for lipids in biological systems is the use of chloroform and methanol in a ratio of 2:1 (v/v) previously reported by Bligh and Dyer (1959), Kofeler et al. (2012), and Folch et al. (1957). According to the Bligh-Dyer method, the solvent ratio of chloroform to methanol to water is 8:4:3 by volume, which provides the most efficient biphasic partitioning of lipids into the organic phase. Due to the solubility and polarity of the different lipid classes, modification of the Bligh and Dyer method using the chloroform-methanol extraction procedure improves the extraction efficiency. In addition to chloroform-methanol as the extraction solvents, isopropanol-hexane mixtures have also been reported for the extraction of prostaglandins (Folch et al. 1957). Although...

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References

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Authors and Affiliations

  1. Metabolomics Australia, School of BioSciences, The University of Melbourne, BioSciences Bldg 2, Parkville, VIC, 3010, Australia

    Thusitha W. T. Rupasinghe

  2. School of BioSciences, The University of Melbourne, BioSciences Bldg 2, Parkville, VIC, 3010, Australia

    Ute Roessner

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  1. Thusitha W. T. Rupasinghe
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  2. Ute Roessner
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Correspondence to Thusitha W. T. Rupasinghe .

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Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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© 2016 Springer Science+Business Media Dordrecht

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Rupasinghe, T.W.T., Roessner, U. (2016). Liquid Extraction: Phase Diagram. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_87-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_87-1

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  • Publisher Name: Springer, Dordrecht

  • Online ISBN: 978-94-007-7864-1

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