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In Vitro Studies on the Antioxidant/Antigenotoxic Potential of Aqueous Fraction from Anthocephalus cadamba Bark

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Perspectives in Cancer Prevention-Translational Cancer Research

Abstract

Cancer is a major public health problem in all parts of the world. With the increasing number of cancer cases worldwide, considerable attention is now being given to natural products for their possible cancer-preventing properties. Increasing evidence suggests that oxidative stress-mediated cardiovascular diseases and cancer-causing oxidation of the DNA molecule can be counteracted by natural antioxidants. The present study was conducted to evaluate the antioxidant/antigenotoxic potential of aqueous fraction from the bark of Anthocephalus cadamba, an important Ayurvedic medicinal plant. The antioxidant activity was assessed by in vitro assays, namely, DPPH (2,2-diphenyl-2-picrylhydrazyl), ABTS 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) assay, reducing power assay, superoxide anion radical scavenging assay and plasmid DNA nicking assay. The antigenotoxicity was studied against 4NQO-induced DNA damage in E. coli PQ37 tester strain using SOS chromotest. The fraction exhibited potent antioxidant activity in all the antioxidant assays. The fraction exhibited 90.77 % activity in DPPH and 97.46 % in ABTS assay at highest tested concentration (200 μg/ml). The fraction showed 67.13 % reduction potential and percent inhibition of 81.00 % in superoxide anion radical scavenging assay at 1,000 μg/ml. The fraction at a concentration of 1,000 μg/ml decreased the SOS-inducing potency(SOSIP) of 4NQO (20 μg/ml) by 21.86 %. Phytochemical analysis of aqueous fraction (AQAB) by UPLC-ESI-QTOF-MS revealed the fraction to be rich in alkaloids.

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Acknowledgements

The authors are thankful to UGC (DRS-SAP), New Delhi, for providing financial assistance. Director of CSIR-IHBT, Palampur, is gratefully acknowledged for providing the lab facility. Upendra Sharma is grateful to CSIR for SRF. We also thank Shiv Kumar for the UPLC analysis.

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Authors and Affiliations

  1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, 143 005, India

    Madhu Chandel & Satwinderjeet Kaur

  2. Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, 176 061, India

    Upendra Sharma, Neeraj Kumar & Bikram Singh

Authors
  1. Madhu Chandel
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  2. Upendra Sharma
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  3. Neeraj Kumar
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  4. Bikram Singh
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  5. Satwinderjeet Kaur
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Correspondence to Satwinderjeet Kaur .

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Editors and Affiliations

  1. Computational Biology & Bioinformatics, University of Kerala, Kariavattom, Trivandrum, Kerala, India

    Perumana R. Sudhakaran

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Chandel, M., Sharma, U., Kumar, N., Singh, B., Kaur, S. (2014). In Vitro Studies on the Antioxidant/Antigenotoxic Potential of Aqueous Fraction from Anthocephalus cadamba Bark. In: R. Sudhakaran, P. (eds) Perspectives in Cancer Prevention-Translational Cancer Research. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1533-2_5

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