Skip to main content
SpringerLink
Log in

Blot Formats: Nucleic Acids

  • Chapter
Book cover Nonradioactive Labeling and Detection of Biomolecules

Part of the book series: Springer Laboratory ((SLM))

  • 125 Accesses

Abstract

Binding between analyte and modified probe, resulting in stable hybrid molecules, is most prominently mediated by specific interaction between complementary purine and pyrimidine bases forming A:T and G:C base pairs.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 69.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Britten RJ, Graham DE, Neufeld BR (1974) Analysis of repeating DNA sequences by reassociation. Meth Enzymol 29: 363–418

    Article  PubMed  CAS  Google Scholar 

  2. Cook AF, Vuocolo E, Brakel CL (1988) Synthesis and hybridization of a series of biotinylated oligonucleotides. Nucleic Acids Res 16: 4077–4095

    Article  PubMed  CAS  Google Scholar 

  3. Davidson EH, Britten RJ (1979) Regulation of gene expression: possible role of repetitive sequences. Science 204: 1052–1059

    Article  PubMed  CAS  Google Scholar 

  4. Gebeyehu G, Rao PY, SooChan P, Simms DA, Klevan L (1987) Novel biotinylated nucleotide analogs for labeling and colorimetric detection of DNA. Nucleic Acids Res 15: 4513–4534

    Article  PubMed  CAS  Google Scholar 

  5. Gillam IC, Tener GM (1986) M-(6-aminohexyl)cytidine and -deoxycytidine nucleotides can be used to label DNA. Anal Biochem 157: 199–207

    Article  PubMed  CAS  Google Scholar 

  6. Haralambidis J, Chai M, Tregear GW (1987) Preparation of base-modified nucleosides suitable for nonradioactive label attachment and their incorporation into synthetic oligonucleotides. Nucleic Acids Res 15: 4857–4876

    Article  PubMed  CAS  Google Scholar 

  7. Hutton JR, Wetmur JG (1973) Length dependence of the kinetic complexity of mouse satellite DNA. Biochem Biophys Res Commun 52: 1148–1155

    Article  PubMed  CAS  Google Scholar 

  8. Huynh Dinh T, Sarfati S, Igolen J, Guesdon JL (1987) Markers for detecting nucleic acids are derived from 2’-desoxy adenosine derivatives. Eur Pat Appl 0254646

    Google Scholar 

  9. Jablonski E, Moomaw EW, Tullis RH, Ruth JL (1986) Preparation of oligonucleotidealkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res 14: 6115–6128

    Article  PubMed  CAS  Google Scholar 

  10. Jablonski E, Ruth JL (1986) Synthesis of oligonucleotide-enzyme conjugates and their use as hybridization probes. DNA 5: 89

    Google Scholar 

  11. Keller GH, Cumming CU, Huang DP, Manak MM, Ting R (1988) A chemical method for introducing haptens onto DNA probes. Anal Biochem 170: 441–450

    Article  PubMed  CAS  Google Scholar 

  12. Keller GH, Manak MM (1989) DNA probes. Stockton Press, New York

    Google Scholar 

  13. Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78: 6633–6637

    Article  PubMed  CAS  Google Scholar 

  14. Marmur J, Doty P (1959) Heterogeneity in DNA. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature 183: 1427–1428

    Google Scholar 

  15. Meinkoth J, Wahl G (1984) Hybridization of nucleic acids immobilized on solid supports. Anal Biochem 138: 267–284

    Article  PubMed  CAS  Google Scholar 

  16. Reisfeld A, Rothenberg JM, Bayer EA, Wilchek M (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem Biophys Res Commun 142: 519–526

    Article  PubMed  CAS  Google Scholar 

  17. Ruth JL, Morgan C, Pasko A (1985) Linker arm nucleotide analogs useful in oligonucleotide synthesis. DNA 4, 93

    Google Scholar 

  18. Tchen P, Fuchs RPP, Sage E, Leng M (1984) Chemically modified nucleic acids as immunodetectable probes in hybridization experiments. Proc Natl Acad Sci USA 81: 3466–3470

    Article  PubMed  CAS  Google Scholar 

  19. Thompson J, Gillespie D (1987) Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate. Anal Biochem 163: 281–291

    Article  PubMed  CAS  Google Scholar 

  20. Urdea MS, Running JA, Horn T, Clyne J, Ku L, Warner BD (1987) A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum. Gene 61: 253–264

    Article  PubMed  CAS  Google Scholar 

  21. Urdea MS, Warner BD, Running JA, Stempien M, Clyne J, Horn T (1988) A comparison of nonradioisotopic hybridization assay method using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes. Nucleic Acids Res 16: 4937–4956

    Article  PubMed  CAS  Google Scholar 

  22. Verdlov ED, Monastyrskaya GS, Guskova LI, Levitan TL, Sheichenko VI, Budowsky EI (1974) Modification of cytidine residues with a bisulfite-O-methylhydroxyl-amine mixture. Biochem Biophys Acta 340: 153–165

    Article  PubMed  CAS  Google Scholar 

  23. Viscidi RP, Connelly CJ, Yolken RH (1986) Novel chemical method for the preparation of nucleic acids for nonisotopic hybridization. J Clin Microbiol 23: 311–317

    PubMed  CAS  Google Scholar 

  24. Wahl GM, Stern M, Stark GR (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci USA 76: 3683–3687

    Article  PubMed  CAS  Google Scholar 

  25. Church G, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995

    Article  PubMed  CAS  Google Scholar 

  26. Rüger R, Höltke H-J, Reischl R, Sagner G, Kessler C (1991) Labeling of specific DNA-sequences with digoxigenin during polymerase chain reaction. In: Rolfs A, Schumacher HC, Marx P (eds) PCR Topics, Springer-Verlag, Berlin

    Google Scholar 

  27. Sambrook J, Fritsch T, Maniatis (1989) Molecular cloning, A Laboratory Manual, (2nd ed), Cold Spring Harbor Laboratory Press

    Google Scholar 

  28. Southern EM (1975) Detection of specific sequences. J Mol Bio 198: 503–517

    Article  Google Scholar 

  29. Thomas PS (1980) Hybridization of denatured RNA and small DNA-fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205

    Article  PubMed  CAS  Google Scholar 

  30. Wood WI, Gitschier J, Lasky LA, Lawn RM (1985) Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci USA 82: 1585–1593

    Article  PubMed  CAS  Google Scholar 

  31. Grunstein M, Hogness DS (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72: 3961–3965

    Google Scholar 

  32. Benton WD, Davis RW (1977) Screening X. gt recombinant dones by hybridization to single plaques in situ. Science 196: 180–182

    Article  PubMed  CAS  Google Scholar 

  33. Voss H, Wirkner U, Jakobi R, Hewitt NA, Schwager C, Zimmermann J, Ansorge W, Pyerins W (1991) Structure of the gene encoding human casein kinase II subunit ß. J Biol Chem 266: 13706–13711

    PubMed  CAS  Google Scholar 

  34. Gibson MA, Sandberg LB, Grosso LE, Cleary EG (1991) Complementary DNA cloning establishes microfibril-associated glycoprotein ( MAGP) to be a discrete component of the elastin-associated microfibrils. J Biol Chem 266: 7596–7601

    Google Scholar 

  35. Schmitz GG, Walter T, Seibl R, Kessler C (1991) Non-radioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal Biochem 192: 222–231

    Article  PubMed  CAS  Google Scholar 

  36. Ali S, Müller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotides specific for simple repeats. Hum Genet 74: 239–243

    Article  PubMed  CAS  Google Scholar 

  37. Arctander P (1988) Comparative studies of avian DNA by restriction fragment length polymorphisms analysis: convenient procedures on blood samples from live birds. J Ornitol 129: 205–216

    Article  Google Scholar 

  38. Epplen JT, Ammer H, Epplen C, Kammerbauer C, Roewer L, Schwaiger W, Steimle V, Zischler H, Albert E, Andreas A, Beyermann B, Meyer W, Buitkamp J, Nanda I, Schmid M, Nürnberg P, Pena SDJ, Pöche H, Sprecher W, Schartl M, Yassouridis A (1991) Oligonucleotide fingerprinting using simple repeat motifs: a convenient, ubiquitously applicable method to detect hypervariability for multiple purposes. In: Burke T, Dolf G, Jeffeys AJ, Wolff R (eds) DNA Fingerprinting: Approaches and Applications, Birkhäuser-Verlag, Basel, 1991, pp 51–69

    Google Scholar 

  39. Epplen JT (1988) On simple repeated GATA/GACA sequences: a critical reappraisal. J Hered 79: 409–417

    PubMed  CAS  Google Scholar 

  40. Epplen JT (1992) The methodology of multilocus DNA fingerprinting using radioactive or nonradioactive oligonucleotide probes specific for simple repeat motifs. In: Chrambach, Dunn, Radola BJ (eds) Adv. Electrophoresis, VCH-Verlag, Weinheim, pp 59–114

    Google Scholar 

  41. Mittermüller J, Hartwig R, Epplen JT, Mönch T, Simon L, Kolb HJ (1991) DNA-Fingerprinting zur Überprüfung des Chimärismus nach allogener Knochenmarkstransplantation. In: Radola BJ (ed) Elektrophorese Forum ‘81, pp 166–171

    Google Scholar 

  42. Nanda I, Deubelbeiss D, Guttenbach M, Epplen JT, Schmid M (1990) Heterogeneitis in the distribution of ( GACA)„ simple repeats in the karyotypes of primates and mouse. Hum Genet 85: 187–194

    Google Scholar 

  43. Nanda I, Schmid M, Epplen JT (1991) In situ hybridization of nonradioactive oligonucleotide probes to chromosomes. In: Adolph KW (ed) Advanced Techniques in Chromosome Research, Marcel Dekker, New York, pp 117–134

    Google Scholar 

  44. Nürnberg P, Roewer L, Neitzel H, Sperling K, Pöpperl A, Hundrieser J, Pöche H, Epplen C, Zischler H, Epplen JT (1989) DNA fingerprinting with the oligonucleotide probe (CAC)5/(GTG)5; somatic stability and germline mutations. Hum Genet 84: 75–78

    Article  PubMed  Google Scholar 

  45. Nürnberg P, Zischler H, Fuhrmann E, Thiel G, Losanova T, Kinzel D, Nisch G, Witkowski R, Epplen JT (1991) Co-amplification of simple repetitive DNA fingerprint fragments and the EGF receptor gene in human gliomas. Genes Chromosomes Cancer 3: 79–88

    Article  PubMed  Google Scholar 

  46. Roewer L, Nürnberg P, Fuhrmann E, Rose M, Prokop O, Epplen JT (1991) Stain analysis using oligonucleotide probes specific for simple repetitive DNA sequences. Forensic Sci Internatl 47: 59–70

    Article  Google Scholar 

  47. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  48. Weising K, Ramser J, Kaemmer D, Kahl G, Epplen JT (1991) Oligonucleotide fingerprinting in plants and fungi. In: Burke T, Jeffreys AJ, Wolff R, Dolf G (eds) DNA-fingerprinting: approaches and applications, Birkhäuser Verlag, Basel, pp 312–331

    Chapter  Google Scholar 

  49. Zischler H, Nanda J, Schäfer R, Schmid M, Epplen JT (1989) Digoxigenated oligonucleotide probes specific for simple repeats in DNA fingerprinting and hybridization in situ. Hum Genet 82: 227–233

    Article  PubMed  CAS  Google Scholar 

  50. Rackwitz HR, Zehetner G, Frischauf A, Lehrach H (1984) Rapid restriction mapping of DNA cloned in lambda phage vectors. Gene 30: 195–200

    Article  PubMed  CAS  Google Scholar 

  51. Rackwitz HR, Zehetner G, Murialdo H, Delius H, Chai JH, Poustka A, Frischauf A, Lehrach H (1985) Analysis of cosmids using linearization by phage lambda terminase Gene 40: 259–266

    CAS  Google Scholar 

  52. Smith HO, Birnstiel ML (1976) A simple method for DNA restriction site mapping. Nucleic Acids Res 3: 2387–2400

    PubMed  CAS  Google Scholar 

  53. Zuber U, Schumann W (1991) Tn5cos: a useful tool for restriction mapping of large plasmide. Gene 103: 69–72

    Article  PubMed  CAS  Google Scholar 

  54. Ansorge W, Sproat B, Stegemann J, Schwager C, Zenke M (1987) Automated DNA Sequencing: Ultrasensitive detection of fluorescent bands during electrophoresis. Nucleic Acids Res: 4593–4602

    Google Scholar 

  55. Beck S (1987) Colorimetric-detected DNA sequencing. Anal Biochem 164:514–520 Beck S, O’Keefe T, Coull J, Köster H (1989) Chemiluminescent detection of DNA: application for DNA sequencing and hybridization. Nucleic Acids Res 17: 5115–5123

    Article  Google Scholar 

  56. Church GM, Kieffer-Higgins S (1988) Multiplex DNA sequencing. Science 240: 185–188

    Article  PubMed  CAS  Google Scholar 

  57. Connell C, Fung S, Heiner C, Bridgeham J, Chakerian V, Heron E, Jones B, Menchen S, Mordan W, Raff M, Recknor M, Smith L, Springer J, Woo S, Hunkapiller M (1987) Automated DNA sequence analysis. Bio Techniques 5: 342–438

    CAS  Google Scholar 

  58. Höltke H-J, Sagner G, Kessler C, Schmitz G (1992) Sensitive chemiluminescent detection of digoxigenin-labeled nucleic acids: a fast and simple protocol and its applications. Bio Techniques 12: 104–113

    Google Scholar 

  59. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467

    Article  PubMed  CAS  Google Scholar 

  60. Tizard R, Cate R, Ramachandran KL, Wysk M, Voyta J, Murphy O, Bronstein J (1990) Imaging of DNA sequences with chemiluminescence. Proc Natl Acad Sci (USA) 87: 4514–4518

    Article  CAS  Google Scholar 

  61. Beck S, O’Keeffe T, Coull JM, Koster H (1989) Chemiluminescent detection of DNA: application for DNA sequencing and hybridization. Nucleic Acids Res 17: 5115–5123

    Article  PubMed  CAS  Google Scholar 

  62. Bronstein I, Juo R-R, Voyta JC, Edwards B (1991) Novel chemiluminescent adamantyl 1,2-dioxetane enzyme substrates. In: Stanley P, Kricka LJ (eds) Bioluminescence and chemiluminescence: Current Status, John Wiley, Chichester, pp 73–82

    Google Scholar 

  63. Creasey A, D’Angio Jr. L, Dunne TS, Kissinger C, O’Keeffe T, Perry-O’Keefe H, Moran LS, Roskey M, Schildkraut I, Sears LE, Slatko B (1991) Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing. Bio Techniques 11: 102–109

    CAS  Google Scholar 

  64. Martin C, Bresnick L, Juo R-R, Voyta JC, Bronstein I (1991) Improved chemiluminescent DNA sequencing. Bio Technique 11: 110–113

    CAS  Google Scholar 

  65. Prober JM, Trainor GL, Dam RJ, Hobbs FW, Robertson CW, Zagursky RJ, Cocuzza AJ, Jensen MA, Baumeister K (1987) A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. Science 238: 336–341

    Article  PubMed  CAS  Google Scholar 

  66. Richterich P, Heller C, Wurst H, Pohl FM (1989) DNA sequencing with direct blotting electrophoresis and colorimetric detection. Bio Techniques 5: 52–59

    Google Scholar 

  67. Richterich P, Church GM (1992) DNA sequencing with direct transfer electrophoresis and nonradioactive detection. Meth Enzymol, Recombinant DNA, Volume H, in press

    Google Scholar 

  68. Tizard R, Cate RL, Ramachandran KL, Wysk M, Voyta JC, Murphy OJ, Bronstein I (1990) Imaging of DNA sequences with chemiluminescence. Proc Natl Acad Sci USA 87: 4514–4518

    Article  PubMed  CAS  Google Scholar 

  69. Beck S (1987) Colorimetric-defected DNA sequencing. Anal Biochem 164: 514–520

    Article  PubMed  CAS  Google Scholar 

  70. Beck S, Pohl FM (1984) DNA sequencing with direct blotting electrophoresis. EMBO J 3: 2905–2909

    PubMed  CAS  Google Scholar 

  71. Pohl FM, Beck S (1987) Direct transfer electrophoresis used for DNA sequencing. Meth Enzymol 155: 250–259

    Article  PubMed  CAS  Google Scholar 

  72. Pohl FM, Feger GM (1992) Boehringer Mannheim Biochemica Colloquium 2: 17–18

    Google Scholar 

  73. Richterich P, Heller C, Wurst H, Pohl FM (1989) DNA sequencing with direct blotting electrophoresis and colorimetric detection. Bio Techniques 7: 52–59

    CAS  Google Scholar 

  74. Smith LM, Sanders JZ, Kaiser RJ, Hughes P, Dodd C, Connell CR, Heiner C, Kent SBH, Hood LE (1986) Fluorescence detection in automated DNA sequence analysis. Nature 321: 674–679

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. Christoph Kessler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Barbara Rüger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cortina Kaletta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Thomas Walter
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jörg T. Epplen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Judith Máthé
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ulrich Zuber
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Wolfgang Schumann
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Gregor Sagner
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Chris S. Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Irena Bronstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Thomas M. Pohl
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Forschungszentrum Penzberg, Boehringer Mannheim GmbH, Nonnenwald 2, W-8122, Penzberg, Germany

    Christoph Kessler

  2. Institut für Biochemie, Ludwig-Maximilians-Universität München, Karlstr. 21, W-8000, München 2, Germany

    Christoph Kessler

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Kessler, C. et al. (1992). Blot Formats: Nucleic Acids. In: Kessler, C. (eds) Nonradioactive Labeling and Detection of Biomolecules. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-00144-8_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-00144-8_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-00146-2

  • Online ISBN: 978-3-662-00144-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation