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The Place of Quantitation in Diagnostic Gastrointestinal Pathology

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Book cover Gastrointestinal Pathology

Part of the book series: Current Topics in Pathology ((CT PATHOLOGY,volume 81))

Abstract

Diagnosis based on histopathological or cytopathological assessment frequently shows considerable interobserver variation. More efficient exchange of information over recent decades has improved diagnostic standards but the subjective nature of the interpretation involved means that the standardisation available in other branches of laboratory medicine cannot easily be achieved. For example despite detailed and widely publicised attempts to achieve more uniform assessment of dysplasia in gastric (Morson et al. 1980; Ming et al. 1984) and in colonic mucosa (Riddell et al. 1983) there is still widespread variation even among histopathologists experienced in this field.

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References

  • Aherne WA, Dunnill MS (1982) Morphometry. Arnold, London

    Google Scholar 

  • Allen DC, Hamilton PW, Watt PCH, Biggart JD (1987) Morphometrical analysis in ulcerative colitis with dysplasia and carcinoma. Histopathology 11:913–926

    PubMed  CAS  Google Scholar 

  • Allen DC, Hamilton PW, Watt PCH, Biggart JD (1988a) Architectural morphometry in ulcerative colitis with dysplasia. Histopathology 12:611–621

    PubMed  CAS  Google Scholar 

  • Allen DC, Connolly NS, Biggart JD (1988b) High iron diamine - alcian blue mucin profiles in benign, premalignant and malignant colorectal disease. Histopathology 13:399–412

    PubMed  CAS  Google Scholar 

  • Armitage NC, Robins RA, Evans DF, Turner DR, Baldwin RW, Hardeastle JD (1985) The influence of tumour cell DNA abnormalities on survival in colorectal cancer. Br J Surg 72:828–830

    PubMed  CAS  Google Scholar 

  • Baak JPA, Oort J (1983) A manual of morphometry in diagnostic pathology. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Baak JPA, Agrafojo Blanco A, Kurver PHJ et al. (1981) Quantitation of borderline and malignant mucinous ovarian tumours. Histopathology 5:353–360

    PubMed  CAS  Google Scholar 

  • Banner BF, Chacho MS, Roseman DL, Coon JS (1987) Multiparameter flow cytometric analysis of colon polyps. Am J Clin Pathol 87:313–318

    PubMed  CAS  Google Scholar 

  • Barlogie B, Drewinko B, Schumann J et al. (1980) Cellular DNA content as a marker of neoplasia in man. Am J Med 69:195–203

    PubMed  CAS  Google Scholar 

  • Behar J, Sheahan DG (1975) Histological abnormalities in reflux oesophagitis. Arch Pathol 99:387–391

    PubMed  CAS  Google Scholar 

  • Bennets R, Stroehlein J, Barlogie B (1979) Ploidy determination by DNA flow cytometry of malignant and benign gastric tissue obtained by endoscopic biopsy. Gastroenterology 76:1099

    Google Scholar 

  • Bezemer PD, Baak JPA, de With C (1977) Discriminant analysis, exemplified with quantitative features of endometrium. Eur J Obstet Gynecol Reprod Biol 7(3):209–214

    PubMed  CAS  Google Scholar 

  • Bohm N, Sandritter W (1975) DNA in human tumours: a cytophotometric study. Curr Top Pathol 60:151–181

    PubMed  CAS  Google Scholar 

  • Bohm N, Sprenger E (1968) Fluorescence cytophotometry: a valuable method for the quantitative determination of nuclear fuelgen-DNA. Histochemie 16:100–118

    PubMed  CAS  Google Scholar 

  • Boon ME, Kurver PJH, Baak JPA, Thompson HT (1981) The application of morphometry in gastric cytological diagnosis. Virchows Arch [A]393:159–164

    CAS  Google Scholar 

  • Bradbury S (1979) Microscopical image analysis: problems and approaches. J Microscopy 115:137–150

    CAS  Google Scholar 

  • Brandtzaeg P, Baklien K, Fäusa O, Hoel PS (1974) Immunohistochemical characterization of local immunoglobulin formation in ulcerative colitis. Gastroenterology 66:1123–1136

    PubMed  CAS  Google Scholar 

  • Brown LJR, Smeeton NC, Dixon MF (1985) Assessment of dysplasia in colorectal adenomas: an observer variation and morphometric study. J Clin Pathol 38:174–179

    PubMed  CAS  Google Scholar 

  • Bussey HJR (1978) Multiple adenomas and carcinomas. In: Bennington JL (ed) Major problems in pathology, vo1 10. The pathogenesis of colorectal cancer. WB Saunders, Philadelphia, pp 72–80

    Google Scholar 

  • Chalkley HW (1943) Methods for the quantitative morphologic analysis of tissues. J Natl Cancer Inst 4:47–53

    Google Scholar 

  • Chalkley HW, Cornfield J, Park H (1949) A method for estimating volume-surface ratios. Science 110:295–297

    PubMed  CAS  Google Scholar 

  • Collins BJ, Elliott H, Sloan JM, McFarland RJ, Love AHG (1985) Oesophageal histology in reflux oesophagitis. J Clin Pathol 38:1265–1272

    PubMed  CAS  Google Scholar 

  • Coon JS, Landay AL, Weinstein RS (1986) Flow cytometric analysis of paraffin-embedded tumours: implications for diagnostic pathology. Hum Pathol 17:435–437

    PubMed  CAS  Google Scholar 

  • Cooper PN, Quirke P, Hardy GJ, Bird CC, Dixon MF (1987) Prediction of the behaviour of smooth muscle tumours of the gastrointestinal tract. J Pathol 152:217A

    Google Scholar 

  • Corrazza GR, Frazzoni M, Gasbarrini G (1984) Jejunal intraepithelial lymphocytes in coeliac disease: are they increased or decreased? Gut 25:158–162

    Google Scholar 

  • Corazza GR, Frazzoni M, Dixon MF, Gasbarrini G (1985) Quantitative assessment of the mucosal architecture of jejunal biopsy specimens: a comparison between linear measurement, stereology and computer aided microscopy. J Clin Pathol 38:765–770

    PubMed  CAS  Google Scholar 

  • Cuvelier CA, Morson BC, Roels HJ (1987) The DNA content in cancer and dysplasia in chronic ulcerative colitis. Histopathology 11:927–939

    PubMed  CAS  Google Scholar 

  • Danno M (1975) Statistical criteria for the cytology of gastric cancer. A proposal of distance index. Acta Cytol 20:446–468

    Google Scholar 

  • Dawson PA, Patel J, Filipe MI (1978) Variations in sialomucins in the mucosa of the large intestine in malignancy: a quantimet and statistical analysis. Histochem J 10:559–572

    PubMed  CAS  Google Scholar 

  • Day DW, Morson BC (1978) The adenoma-carcinoma sequence. In: Bennington JL (ed) Major problems in pathology, vol 10. The pathogenesis of colorectal cancer. WB Saunders, Philadelphia, pp 58–71

    Google Scholar 

  • Deinlein E, Schmidt H, Riemann J-F, Gra El-Pietrusky R, Hornstein OP (1983) DNA flow cytometric measurements in inflammatory and malignant human gastric lesions. Virchows Archiv [A]402:185–193

    CAS  Google Scholar 

  • Dhesi I, Marsh MN, Kelly C, Crowe P (1984) Morphometric analysis of small intestinal mucosa. Virchows Arch [A]403:173–180

    CAS  Google Scholar 

  • Dukes CE (1932) The classification of cancer of the rectum. J Pathol Bact 35:323–332

    Google Scholar 

  • Dundas SAC, Kay R, Beck S, Cotton DWK, Coup aJ, Slater DN, Underwood JCE (1987) Can histopathologists reliably assess dysplasia in inflammatory bowel disease? J Clin Pathol 40:1282–1286

    PubMed  CAS  Google Scholar 

  • Dunnill MS, Whitehead R (1972) A method for the quantitation of small intestinal biopsy specimens. J Clin Pathol 25:243–246

    PubMed  CAS  Google Scholar 

  • Eide TJ (1986) Prevalence and morpological features of adenomas of the large intestine in individuals with and without colorectal carcinoma. Histopathology 10:111–118

    PubMed  CAS  Google Scholar 

  • Elias H, Hyde DM, Mullens RS, Lambert FC (1981) Colonic adenomas: stereology and growth mechanisms. Dis Colon Rectum 24:331–342

    PubMed  CAS  Google Scholar 

  • Enblad P, Glimelius B, Bengtssen A, Ponten J, Pahlmen L (1985) DNA content in carcinoma of the rectum and rectosigmoid. Acta Pathol Microbiol Immunol Scand [A]93:277–284

    CAS  Google Scholar 

  • Ferguson A, Murray D (1971) Quantitation of intraepithelial lymphocytes in human jejunum. Gut 12:989–994

    Google Scholar 

  • Ferguson A, Ziegler K (1986) Intraepithelial lymphocyte mitoses in a jejunal biopsy correlates with intraepithelial lymphocyte count irrespective of diagnosis. Gut 27:675–679

    PubMed  CAS  Google Scholar 

  • Filipe MI (1969) Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut 10:577–586

    PubMed  CAS  Google Scholar 

  • Filipe MI (1984) Mucins of normal, premalignant and malignant colonic mucosa. In: Wolman SR, Mastromarina AJ (eds) Progress in cancer research and therapy, vol 29. Raven, New York, pp 237–251

    Google Scholar 

  • Forsslund G, Cedermark B, Ohman U, Erhardt K, Zetterberg A, Auer G (1984) The significance of DNA distribution pattern in rectal carcinoma. A preliminary study. Dis Colon Rectum 27:579–584

    PubMed  CAS  Google Scholar 

  • Fozard JBJ, Quirke P, Dixon MF, Giles GR, Bird CC (1986) DNA aneuploidy in ulcerative colitis. Gut 27:1414–1418

    PubMed  CAS  Google Scholar 

  • Gardiner GW, Goldberg R, Currie D, Murray D (1984) Colonic carcinoma associated with an abnormal collagen table. Cancer 54:2973–2977

    PubMed  CAS  Google Scholar 

  • Giard RWM, Hermans J, Ruiter DJ, Hoedemaeker PHJ (1985) Variations in histopathological evaluation of non-neoplastic mucosal abnormalities: assessment and clinical significance. Histopathology 9:535–541

    PubMed  CAS  Google Scholar 

  • Goh HS, Jass JR (1986) DNA content and the adenoma-carcinoma sequence in the colorectum. J Clin Pathol 39:387–392

    PubMed  CAS  Google Scholar 

  • Goh HS, Jass JR, Atkin WS, Cuzick J, Northover JMA (1987) Value of flow cytometric determination of ploidy as a guide to prognosis in operable rectal cancer. Int J Colorect Dis 2:17–21

    CAS  Google Scholar 

  • Guix M, Skinner JM, Whitehead RW (1979) Measuring intraepithelial lymphocytes, surface area and volume of lamina propria in the jejunal mucosa of coeliac patients. Gut 20:275–278

    PubMed  CAS  Google Scholar 

  • Hamilton PW, Allen DC, Watt PCH, Patterson CC, Biggart JD (1987a) Classification of normal colorectal mucosa and adenocarcinoma by morphometry. Histopathology 11:901–911

    PubMed  CAS  Google Scholar 

  • Hamilton PW, Collins JSA, Watt PCH, Sloan JM, Love AHG (1987b) Morphometric assessment of oesophagitis in pinch biopsies and correlation with ambulatory pH data. J Pathol 151:78A

    Google Scholar 

  • Hamilton PW, Watt PCH, Collins JSA, Sloan JM, Love AHG (1987c) Objective grading of gastritis and duodenitis: a comparison of two methods. J Pathol 152–228A

    Google Scholar 

  • Hamilton PW, Watt PCH, Allen DC (1988) A morphometrical assessment of transitional mucosa in the colon. Histopathology 13:519–530

    PubMed  CAS  Google Scholar 

  • Hammarberg C, Slezak P, Tribukait B (1984) Early detection of malignancy in ulcerative colitis. A flow-cytometric DNA study. Cancer 53:291–295

    PubMed  CAS  Google Scholar 

  • Heatley RV, James PD (1979) Eosinophils in the rectal mucosa. A simple method of predicting the outcome of ulcerative proctocolitis? Gut 20:787–791.

    PubMed  CAS  Google Scholar 

  • Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31:1333–1335

    PubMed  CAS  Google Scholar 

  • Hedley DW, Friedlander ML, Taylor IW (1985) Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry 6:327–333

    PubMed  CAS  Google Scholar 

  • Hywel-Jones J, Lcnnard-Joncs JE, Morson BC (1973) Numerical taxonomy and discriminant analysis applied to non-specific colitis. Q J Med 42:715–732

    Google Scholar 

  • Inokuchi K, Kodama Y, Sasaki O, KamegawaT, Okamura T (1983) Differentiation of growth patterns of early gastric carcinoma determined by cytophotometric DNA analysis. Cancer 51:1138–1141

    PubMed  CAS  Google Scholar 

  • Inui N. Oata K (1965) DNA content of human tumour cell nucleus: a study on gastric carcinoma with special reference to its histological features. Gann 56:567–574

    PubMed  Google Scholar 

  • Ismail-Beigi F, Horton PF, Pope CE (1970) Histological consequences of gastroesophageal reflux in man. Gastroenterology 58:163–174

    PubMed  CAS  Google Scholar 

  • Jarvis LR, Whitehead R (1985) Morphometric analysis of gastric dysplasia. J Pathol 47:133–138

    Google Scholar 

  • Jarvis LR, Dent J, Whitehead R (1985) Morphometrie assessment of reflux oesophagitis in fibreoptic biopsy specimens. J Clin Pathol 38:44–48

    PubMed  CAS  Google Scholar 

  • Jarvis LR, Graff PS, Whitehead R (1987) Correlation of nuclear ploidy with histology in adenomatous polyps of colon. J Clin Pathol 40:26–33

    PubMed  CAS  Google Scholar 

  • Jass JR, Morson BC (1987) Reporting colorectal carcinoma. J Clin Pathol 40:1016–1023

    PubMed  CAS  Google Scholar 

  • Jass JR, Love SB, Northover JMA (1987) A new prognostic classification of rectal cancer. Lancet 1:1303–1306

    PubMed  CAS  Google Scholar 

  • Jenkins D, Goodall A, Gillet FR, Scott BB (1985) Defining duodenitis: quantitative histological study of mucosal responses and their correlations. J Clin Pathol 38:1119–1126

    PubMed  CAS  Google Scholar 

  • Jenkins D, Goodall A, Scott BB (1986) Tlymphocyte populations in normal and coeliac small intestinal mucosa defined by monoclonal antibodies. Gut 27:1330–1331

    PubMed  CAS  Google Scholar 

  • Jenkins D, Goodall A, Drew K, Scott BB (1988) What is colitis? Statistical approach to distinguishing clinically important inflammatory change in rectal biopsy specimens. J Clin Pathol 41:72–79

    PubMed  CAS  Google Scholar 

  • Kayser KK, Modlinger F, Postl K (1985) Quantitative low-resolution analysis of colon mucosa. Anal Qant Cytol Histol 7:205–212

    CAS  Google Scholar 

  • Keren DF, Appelman HD, Dobbins WO et al. (1984) Correlation of histopathologic evidence of disease activity with the presence of immunoglobulin-containing cells in the colons of patients with inflammatory bowel disease. Hum Pathol 15:757–763

    PubMed  CAS  Google Scholar 

  • Kiefer G, Sandritter W (1976) DNA and the cell cycle. Beitr Pathol 158:332–362

    PubMed  CAS  Google Scholar 

  • Konishi F, Morson BC (1982) Pathology of colorectal adenomas: a colonoscopic survey. J Clin Pathol 35:830–841

    PubMed  CAS  Google Scholar 

  • Kreuning J, Bosman FT, Kuiper G, Wal AM, Lindeman J (1978) Gastric and duodenal mucosa in “healthy” individuals. An endoscopic and histopathological study of 50 volunteers. J Clin Pathol 31:69–77

    PubMed  CAS  Google Scholar 

  • Langley FA, Baak JPA, Oort J (1983) Diagnosis making: error sources. In: Baak JPA, Oort J (eds) A manual of morphometry in diagnostic pathology. Springer, Berlin Heidelberg New York, pp 6–14

    Google Scholar 

  • Leigh RJ, Marsh MN, Crowe P, Kelly C, Garner V, Gordon D (1985) Studies of intestinal lymphoid tissue IX. Dose dependent gluten induced lymphoid infiltration of coeliac jejunal epithelium. Scand J Gastroenterol 20:715–719

    PubMed  CAS  Google Scholar 

  • Lennard-Jones JE, Morson BC, Ritchie JK, Williams CB (1983) Cancer suveillance in ulcerative colitis. Experience over 15 years. Lancet 11:149–152

    Google Scholar 

  • Leuchtenberger C, Leuchtenberger R, Davis A (1954) A microspectrophotometric study of the deoxyribonucleic acid (DNA) content in cells of normal and malignant human tissues. Am J Pathol 30:65–85

    PubMed  CAS  Google Scholar 

  • Lev R. Lance P, Camara P (1985) Histochemical and morphologic studies of mucosa bordering rectosigmoid carcinomas: comparisons with normal, diseased and malignant colonic epithelium. Hum Pathol 16:151–161

    PubMed  CAS  Google Scholar 

  • Lindstrom CG (1976) “Collagenous colitis” with watery diarrhoea: a new entity? Pathologia Europaea 11:87–89

    PubMed  CAS  Google Scholar 

  • Lofberg R, Tribukait B, Ost A, Brostrom O. Reichard H (1987) Flow cytometric DNA analysis in longstanding ulcerative colitis: a method of prediction of dysplasia and carcinoma development? Gut 28: 1100–1106

    PubMed  CAS  Google Scholar 

  • Lovett EJ, Schnitzer B, Keren DF, Flint A, Hudson JL, McClatchey KD (1984) Application of flow cytometry to diagnostic pathology. Lab Invest 50:115–153

    PubMed  CAS  Google Scholar 

  • Lowe J, Kent J. Armitage NC, Ballantyne KC, Hardcastle JD (1986) Nuclear morphometry and nuclear DNA content in rectal carcinoma. In: Fourth International Symposium on Morphometry in Morphological Diagnosis. The Royal Society of Medicine, London, p 19

    Google Scholar 

  • Macartney JC, Camplejohn RS (1986) DNA flow cytometry of histological material from dysplastic lesions of human gastric mucosa. J Pathol 150:113–118

    PubMed  CAS  Google Scholar 

  • Macartney JC, Camplejohn RS. Powell G (1986) DNA flow cytometry of histological material from human gastric cancer. J Pathol 148:273–277

    PubMed  CAS  Google Scholar 

  • MacDermott RP (1986) Symposium on cell-mediated immunity in human disease. Part II. Cell-mediated immunity in gastrointestinal disease. Hum Pathol 17:219–233

    PubMed  CAS  Google Scholar 

  • Malizia G, Trejdosiewicz LK, Wood GM, Howdle PD, Janossy G, Losowsky MS (1985) The microenvironment of coeliac disease: T cell phenotypes and expression of the T2 ‘T blast’ antigen by small bowel lymphocytes. Clin Exp Immunol 60:437–446

    PubMed  CAS  Google Scholar 

  • Marsh MN (1980) Studies of intestinal lymphoid tissue III. Quantitative analysis of intraepithelial lymphocytes in the small intestine of human control subjects and of patients with coeliac spruce. Gastroenterology 79:481–492

    PubMed  CAS  Google Scholar 

  • Marsh MN (1982) Studies of intestinal lymphoid tissue IV — the predictive value of raised mitotic indices among jejunal epithelial lymphocytes in the diagnosis of gluten sensitive enteropahty. J Clin Pathol 35:517–525

    PubMed  CAS  Google Scholar 

  • Marsh MN (1985) Functional and structural aspects of the epithelial lymphocyte with implications for coeliac disease and tropical sprue. Scand J Gastroenterol [Suppl] 114:55–75

    CAS  Google Scholar 

  • Marsh MN (1986) Measurement of intra-epithelial lymphocytes. Gut 27:1516–1517

    PubMed  CAS  Google Scholar 

  • Maskens AP (1979) Histogenesis of adenomatous polyps in the human large intestine. Gastroenterology 77:1245–1251

    PubMed  CAS  Google Scholar 

  • McKinley M, Budman D, Caccese W, Weissman G, Scheidt N, Kahn E, Bronco R (1985) Evaluation of colonic neoplasia by flow cytometry of endoscopic biopsies. Am J Gastroenterol 80:47–49

    PubMed  CAS  Google Scholar 

  • Meier-Rüge W (1974) Hirschsprung’s disease: its aetiology, pathogenesis and differential diagnosis. In: Grundmann E, Kirsten WH (eds) Curr Top Pathol 59:131–179

    Google Scholar 

  • Melamed M, Enker WE, Banner P, Janov AJ, Kessler G, Darzynkiewicz Z (1986) Flow cytometry of colorectal carcinoma aith three year follow-up. Dis Colon Rectum 29:184–186

    PubMed  CAS  Google Scholar 

  • Melville DM, Jass JR, Shepherd NA (1987) DNA aneuploidy as a marker of neoplastic change in ulcerative colitis. J Pathol 152:215A

    Google Scholar 

  • Ming S-C, Bajtai A, Correa P et al. (1984) Gastric dysplasia. Significance and pathologic criteria. Cancer 54:1794–1801

    PubMed  CAS  Google Scholar 

  • Morson BC, Dawson IMP (1979) Gastro-intestinal pathology, 2nd edn Blackwell Scientific, London

    Google Scholar 

  • Morson BC, Sobin LH, Grundmann E, Johansen A, Nagayo T, Serch-Hanssen A (1980) Pre-cancerous conditions and epithelial dysplasia in the stomach. J Clin Pathol 33:711–721

    PubMed  CAS  Google Scholar 

  • Mukada T, Sasano N, Sato E (1978) Evaluation of esophageal dysplasia by cytofluorometric analysis. Cancer 41:1399–1404

    PubMed  CAS  Google Scholar 

  • Nakamura K, Nakayo T, Sato E, Sasano N (1974) Ultramorphometry of the nucleus of human rectal cancer compared with normal mucosal gland. Tohoku J Exp Med 112:177–188

    PubMed  CAS  Google Scholar 

  • Nielsen VT, Vetner M, Harslof E (1980) Collagenous colitis. Histopathology 4:83–86

    PubMed  CAS  Google Scholar 

  • Nogaller AM, Nikonenko TN, Avirina TK (1983) Clinico-morphological and morphometric characteristics of chronic colitis. Klin Med (Mosk) 61:82–88

    CAS  Google Scholar 

  • Ochlert W, Keller P, Henke M, Strauch M (1979) Gastric mucosal dysplasia: what is its clinical significance? Front Gastrointest Res 4:173–182

    Google Scholar 

  • Oja E, Collan Y (1983) Basic principles of image analysis by computer. Acta Stereol 2:250–261

    Google Scholar 

  • Owen DA (1984) Gastritis and duodenitis. In: Appleman HD (ed) Pathology of the oesophagus, stomach and duodenum. Churchill Livingstone, Edinburgh, pp 37–43

    Google Scholar 

  • Painter NS (1964) The aetiology of diverticulosis of the colon with special reference to the action of certain drugs on the behaviour of the colon. Ann R Coll Surg Engl 34:98–119

    PubMed  CAS  Google Scholar 

  • Painter NS, Burkitt DP (1975) Diverticular disease of the colon is a 20th century problem. Clin Gastroenterol 4:3–21

    PubMed  CAS  Google Scholar 

  • Parl FF, Lev R, Thomas E, Pitchumuni CS (1979) Histologic and morphometric study of chronic gastritis in alcoholic patients. Hum Pathol 10:45–56

    PubMed  CAS  Google Scholar 

  • Petrova AS, Subrichina GN, Tschistjakova OV. Lukina TA, Weiss H, Wildner G (1980) Flow cytofluorometry. cytomorphology and histology in gastric carcinoma. Oncology 37:318–324

    PubMed  CAS  Google Scholar 

  • Phelps PC, Toker C. Trump BF (1979) Surface ultrastructure of normal, adenomatous and malignant epithelium from human colon. Scan Electron Microsc 3:169–176

    PubMed  Google Scholar 

  • Quirkc P, Dyson JED (1986) Flow cytometry: methodology and applications in pathology. J Pathol 149: 79–87

    Google Scholar 

  • Quirke P, Dyson JED, Dixon MF, Bird CC. Joslin CAF (1985a) Heterogeneity of colorectal adenocarcinomas evaluated by flow cytometry and histopathology. Br J Cancer 51:99–106

    PubMed  CAS  Google Scholar 

  • Quirke p, Fozard JBJ, Dixon MF, Dyson JED, Giles GR, Bird CC (1985b) DNA aneuploidy in colorectal adenomatous polyps with and without synchronous carcinoma. J Pathol 146:267A

    Google Scholar 

  • Quirke P, Dixon MF, Fozard JBJ, Day D, Talbot IC, Bird CC (1987a) DNA aneuploidy in familial polyposis coli: a flow cytometric study. J Pathol 151:76A

    Google Scholar 

  • Quirke P, Dixon MF, Clayden AD, Durdey P, Dyson JED, Williams NS, Bird CC (1987b) Prognostic significance of DNA aneuploidy and cell proliferation in rectal adenocarcinomas. J Pathol 151:285–291

    PubMed  CAS  Google Scholar 

  • Riddell RH (1976) The precarcinomatous phase of ulcerative colitis. In: Morson BC (ed) Current topics in pathology. Pathology of the gastrointestinal tract. Springer Berlin Heidelberg, New York, pp 179–219

    Google Scholar 

  • Riddell RH, Goldman H, Ransohoff DF et al. (1983) Dysplasia in inflammatory bowel diseases: standardized classification with provisional clinical applications. Hum Pathol 14:931–968

    PubMed  CAS  Google Scholar 

  • Rosekrans PCM, Meijer CJLM, Cornelisse CJ, van der Wal AM, Lindemann J (1980a) Use of morpohometry and immunohistochemistry of small intestinal biopsy specimens in the diagnosis of food allergy. J Clin Pathol 33:125–130

    PubMed  CAS  Google Scholar 

  • Rosekrans PCM, Meijer CJLM, van der Wal AM, Cornelisse CJ, Lindeman J (1980b) Immunoglobulin containing cells in inflammatory bowel disease of the colon: a morphometric and immunohistochemical study. Gut 21:941–947

    PubMed  CAS  Google Scholar 

  • Rosekrans PCM, Meijer CJLM, Polanco I, Mearin ML, van der Wal AM, Lindeman J (1981a) Long term morphological and immunohistochemical observations on biopsy specimens of small intestine from children with gluten sensitive enteropathy. J Clin Pathol 34: 138–144

    PubMed  CAS  Google Scholar 

  • Rosekrans PCM, Lindeman J, Meijer CJLM (1981b) Quantitative immunological and immunohistochemical findings in jejunal biopsy specimens in giardiasis. Virchows Arch [A]393:145–151

    CAS  Google Scholar 

  • Rubin CE, Brandbort LL, Phelps DC, Taylor HC (1960) Studies of celiac disease. I. Gastroenterology 38:28–49

    PubMed  CAS  Google Scholar 

  • Rubio CA, Johansson C, Kock Y (1982a) A quantitative method of estimating inflammation in the rectal mucosa. II. Normal limits in symptomatic patients. Scand J Gastroenterol 17:1077–1081

    PubMed  CAS  Google Scholar 

  • Rubio CA, Johansson C, Kock Y (1982b) A quantitative method of estimating inflammation in the rectal mucosa. Ill Chronic ulcerative colitis. Scand J Gastroenterol 17:1083–1087

    PubMed  CAS  Google Scholar 

  • Rubio CA, Johansson C, Uribe A, Kock Y (1984) A quantitative method of estimating inflammation in the rectal mucosa. IV. Ulcerative colitis in remission. Scand J Gastroenterol 19:525–530

    PubMed  CAS  Google Scholar 

  • Saffos RO, Rhatigan RM (1977) Benign (non-polypoid) mucosal changes adjacent to carcinoma of the colon. Hum Pathol 8:441–449

    PubMed  CAS  Google Scholar 

  • Sanderson IR, Slavin G, Walker-Smith JA (1985) Density of mucosal mast cells in the lamina propria of the colon and terminal ileum of children. J Clin Pathol 38:771–773

    PubMed  CAS  Google Scholar 

  • Sarin SK, Malhotra V, Gupta SS, Karol A, Gaur SK, Anand BS (1987) Significance of eosinophil and mast cell counts in rectal mucosa in ulcerative colitis. A prospective controlled study. Dig Dis Sci 32:363–367

    PubMed  CAS  Google Scholar 

  • Sassi I, Faravelli A, Freschi M, Ferrari AM, Cantaboni A (1986) Planimetric grading of dysplasia in colorectal adenomas: a step forward? In: Fourth International Symposium on Morphometry in Morphological Diagnosis. The Royal Society of Medicine, London, p 78

    Google Scholar 

  • Sato E, Goto N, Nakamura T (1981) Nuclear ultrastructure in carcinoma, adenoma, Peutz-Jeghers polyp and dysplasia of the large bowel: a morphometric analysis. Gann 72:245–253

    PubMed  CAS  Google Scholar 

  • Schutte B, Reynders MMJ, Bosman FT, Blijham GH (1985) Flow cytometric determination of DNA ploidy level in nuclei isolated free from paraffin-embedded tissue. Cytometry 6:26–30

    PubMed  CAS  Google Scholar 

  • Scott BB, Goodall A, Stephenson P, Jenkins D (1983) Rectal mucosal plasma cells in inflammatory bowel disease. Gut 24:519–524

    PubMed  CAS  Google Scholar 

  • Scott NA, Rainwater LM, Vieand HS, Weiland LH, Pemberton JH, Beart RW, Lieber NM (1987) The relative prognostic value of flow cytometric DNA analysis and conventional clinicopathologic criteria in patients with operable rectal carcinoma. Dis Colon Rectum 30:513–520

    PubMed  CAS  Google Scholar 

  • Seefeld U, Kreys GJ, Siebermann RE, Blum AL (1977) Esophageal histology in gastroesophageal reflux. Morphometric findings on suction biopsies. Dig Dis Sci 22:956–964

    CAS  Google Scholar 

  • Selby WS, Janossy G, Jewell DP (1981a) Immunohistological characterisation of intraepithelial lymphocytes of the human gastrointestinal tract. Gut 22:169–176

    PubMed  CAS  Google Scholar 

  • Selby WS, Janossy G, Goldstein G, Jewell DP (1981b) T lymphocyte subsets in the human intestinal mucosa: the distribution and relationship to MHC-derived antigens. Clin Exp Immunol 44:453–458

    PubMed  CAS  Google Scholar 

  • Selby WS, Janossy G, Bofill M, Jewell DP (1983) Lymphocyte subpopulations in the human small intestine. The findings in normal mucosa and in the mucosa of patients with adult coeliac disease. Clin Exp Immunol 52:219–228

    PubMed  CAS  Google Scholar 

  • Seldenrijk CA, Hendriks G, Baak JPA, Van Spreejuwel JP, Smeulders AWM, Meuwissen SGM, Meijer CJLM (1986) Plasma cell counting in colonic biopsies. In: Fourth International Symposium on Morphometry in Morphological Diagnosis. The Royal Society of Medicine, London, p 79

    Google Scholar 

  • Shiner M, Doniach I (1960) Histopathologic studies in steatorrhoea. Gastroenterology 38:419–440

    PubMed  CAS  Google Scholar 

  • Slavin G, Sowter C, Robertson K, McDermott S, Paton K (1980) Measurement in jejunal biopsies by computer aided microscopy. J Clin Pathol 33:254–261

    PubMed  CAS  Google Scholar 

  • Spence RAJ, Sloan JM, Johnston GW, Greenfield A (1983) Oesophageal mucosal changes in patients with varices. Gut 24:1024–1029

    PubMed  CAS  Google Scholar 

  • Spence RAJ, Sloan JM, Johnston GW (1984) Histologic factors of the oesophageal transection ring as clues to the pathogenesis of bleeding varices. Surg Gynecol Obstet 159:253–259

    PubMed  CAS  Google Scholar 

  • Sprenger E, Witte S, Schaden M (1974) The differential diagnostic role of nuclear DNA content in gastric cytology. In: Sprenger E, Witte S, Schaden M (eds) Early gastric cancer. Springer, Berlin Heidelberg New York, pp 154–158

    Google Scholar 

  • Stich HF, Florian SF, Emson HE (1960) The DNA content of tumour cells. I. Polyps and adenocarcinomas of the large intestine of man. J Natl Cancer Inst 24:471–482

    PubMed  CAS  Google Scholar 

  • Strickland RG, Husby G, Black WC, Williams RC Jr (1975) Peripheral blood and intestinal lymphocyte sub-populations in Crohn’s disease. Gut 16:847–853

    PubMed  CAS  Google Scholar 

  • Sugimachi K, Inokuchi K, Matsuura H, Okamura T, Sasaki O (1983) Cytophotometric DNA analysis of early esophageal carcinoma. Jpn J Surg 13:37–41

    PubMed  CAS  Google Scholar 

  • Surawicz CM, Belie L (1984) Rectal biopsy helps to distinguish acute self-limited colitis from idiophatic inflammatory bowel disease. Gastroenterology 86:104–113

    PubMed  CAS  Google Scholar 

  • Teodori L, Capurso L, Cordelli E et al. (1984) Cytometrically determined relative DNA content as an indicator of neoplasia in gastric lesions. Cytometry 5:63–70

    PubMed  CAS  Google Scholar 

  • Thompson EM, Price AB, Altman DG, Sowter C, Slavin G (1985) Quantitation in inflammatory bowel disease using computerised interactive image analysis. J Clin Pathol 38:631–638

    PubMed  CAS  Google Scholar 

  • Thurlbeck WM, Benson JA, Dudley HR (1960) The histopathological changes of sprue and their significance. Am J Clin Pathol 34:108–117

    PubMed  CAS  Google Scholar 

  • Tosi P, Luzi P, Baak JPA, Miracco C, Vindigni C, Lio r, Barbini P (1987) Gastric dysplasia: a stereological and morphometrical assessment. J Pathol 152:83–94

    PubMed  CAS  Google Scholar 

  • Toukan AU, Kamal MF, Amr SS, Arnaout MA, Abu-Romiyeh AS (1985) Gastro-duodenal inflammation in patients with non-ulcer dyspepsia. A controlled endoscopic and morphometric study. Dig Dis Sci 30:313–320

    PubMed  CAS  Google Scholar 

  • Underwood EE (1970) Quantitative Steriology. Addison Wesley, Reading Mass

    Google Scholar 

  • Van den Ingh HF, Griffioen G, Cornelisse CJ (1985) Folw cytometric detection of aneuploidy in colorectal adenomas. Cancer Res 45:3392–3397

    PubMed  Google Scholar 

  • Van Spreeuwel JP, Lindeman J, Meijer CJLM (1985) A quantitative study of immunoglobulin containing cells in the differential diagnosis of acute colitis. J Clin Pathol 38:774–777

    PubMed  Google Scholar 

  • Van Spreeuwel JP, Meijer CJLM, Rosekrans PCM, Lindeman J (1986) Immunoglobulin containing cells in gastrointestinal pathology - diagnostic applications. Pathol Annu 21:295–310

    PubMed  Google Scholar 

  • Watson AJ, Wright NA (1974) Morphology and cell kinetics of the jejunal mucosa in untreated patients with coeliac disease. Clin Gastrenterol 3:11–31

    CAS  Google Scholar 

  • Watt PCH, Collins JSA, Hamilton PW, Sloan JM, Love AHG (1987) A quantitative assessment of mucosal inflammation in patients with non-ulcer dyspepsia, duodenitis and duodenal ulcer. J Pathol 152:228 A–229 A

    Google Scholar 

  • Weibel ER (1963) Principles and methods for morphometric study of the lung and other organs. Lab Invest 12:131–155

    PubMed  CAS  Google Scholar 

  • Weibel ER (1969) Stereological principles for morphometry in electron microscopic cytology. Int Rev Cytol 26:235–302

    PubMed  CAS  Google Scholar 

  • Weibel ER (1979) Stereological methods, vol I. Practical methods for biological morphometry. Academic, London

    Google Scholar 

  • Weinreb M, Zajicek G, Levij IS (1984) Discrimination betweeen normal and malignant gastric epithelial cells by computer image analysis. Anal Quant Cytol 6:206–210

    PubMed  CAS  Google Scholar 

  • Weinstein WM, Bogoch ER, Bowes KL (1975) The normal human oesophageal mucosa: a histological reappraisal. Gastroenterology 68:40–44

    PubMed  CAS  Google Scholar 

  • Weiss H, Gutz H-J, Wildner GP, Ebeling K, Schmidt W (1980) Characterisation of chronic atrophic gastritis by means of pulse cytophotometry. Acta Pathol Microbiol Scand [B] [Suppl] 274:412–416

    Google Scholar 

  • Whitehead F, Jarvis LR, Skinner JM, Whitehead R (1985) Ploidy studies in adenomatous polyps of the colon J Clin Pathol 38:1261–1264

    PubMed  CAS  Google Scholar 

  • Whitehead R (1985) Mucosal biopsy of the gastrointestinal tract. WB Saunders, London

    Google Scholar 

  • Whitehead R (1986) Measurement of intraepithelial lymphocytes. Gut 27:1399

    PubMed  CAS  Google Scholar 

  • Whitehead R, Roca M, Meikle DD, Skinner J, Truelove SC (1975) The histological classification of duodenitis in fibre-optic biopsy specimens. Digestion 13:129–136

    PubMed  CAS  Google Scholar 

  • Whiteway J, Morson BC (1985) Elastosis in diverticular disease of the sigmoid colon. Gut 26:258–266

    PubMed  CAS  Google Scholar 

  • Williams GT (1985) Commentary. Transitional mucosa of the large intestine. Histopathology 9:1237–1243

    PubMed  CAS  Google Scholar 

  • Williams GT, Rhodes J (1987) Collagenous colitis: disease or diversion? Br Med J 294:855–856

    CAS  Google Scholar 

  • Wolley RC, Schreiber K, Koss LG, Karas M, Sherman A (1982) DNA distribution in human colon carcinomas and its relationship to clinical behaviour. J Natl Cancer Inst 69:15–22

    PubMed  CAS  Google Scholar 

  • Wright SG, Tomkins AM (1978) Quantitative histology in giardiasis. J Clin Pathol 31:712–716

    PubMed  CAS  Google Scholar 

  • Wyatt JI, Quirke P, Ward D, Johnson DJ, Dixon MF, Bird CC (1987) Comparison of histopathological and flow cytometric parameters in the prediction of prognosis in gastric cancer. J Pathol 152:233 A

    Google Scholar 

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Authors
  1. J. M. Sloan
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  2. D. C. Allen
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  3. P. W. Hamilton
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  4. P. C. H. Watt
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Editors and Affiliations

  1. Department of Pathology, College of Medicine, University of Wales, CF4 4XN, Cardiff, UK

    Geraint T. Williams B. Sc., M. D., M. R. C. P., M. R. C. Path.,

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Sloan, J.M., Allen, D.C., Hamilton, P.W., Watt, P.C.H. (1990). The Place of Quantitation in Diagnostic Gastrointestinal Pathology. In: Williams, G.T. (eds) Gastrointestinal Pathology. Current Topics in Pathology, vol 81. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74662-8_8

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  • DOI: https://doi.org/10.1007/978-3-642-74662-8_8

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