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Isolation and Purification of the Nicotinic Acetylcholine Receptor from Torpedo Electric Organ

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Membrane Proteins

Abstract

The nicotinic acetylcholine receptor (nAcChR) can be extracted and purified to homogeneity from fish electroplaques. These organs are regarded as modified skeletal muscles with a very rich innervation of the cholinergic type. Those from the electric ray Torpedo are the richest known source of nAcChR. They contain 12,000–15,000 receptors per μm2 on the postsynaptic membrane.

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© 1981 Springer-Verlag Berlin Heidelberg

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Fulpius, B.W., Bersinger, N.A., James, R.W., Schwendimann, B. (1981). Isolation and Purification of the Nicotinic Acetylcholine Receptor from Torpedo Electric Organ. In: Azzi, A., Brodbeck, U., Zahler, P. (eds) Membrane Proteins. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68077-9_7

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  • DOI: https://doi.org/10.1007/978-3-642-68077-9_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-10749-1

  • Online ISBN: 978-3-642-68077-9

  • eBook Packages: Springer Book Archive

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