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Zellbiologische Grundlagen der Zell- und Gewebekultur

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Zell- und Gewebekultur

Zusammenfassung

Im Unterschied zur mikrobiellen Kultur, wo die einzelne Zelle bereits einen Organismus für sich darstellt, wird in der Säugerzellkultur eine Zelle aus dem Gewebeverband herausgelöst und unter In-vitro-Bedingungen gehalten. Für eine erfolgreiche Zell- und Gewebekultur mussten zwei entscheidende Probleme gelöst werden: (1) Populationen von Zellen mussten aus einigen wenigen Zellen herangezüchtet werden können, und (2) anschließend über mehrere Generationen am Leben erhalten werden (Abschn. 16.1).

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Gstraunthaler, G., Lindl, T. (2013). Zellbiologische Grundlagen der Zell- und Gewebekultur. In: Zell- und Gewebekultur. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35997-2_4

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