Skip to main content

Qualitätskontrolle und Cell Banking

  • Chapter
  • 13k Accesses

Zusammenfassung

Die Kultivierung humaner oder tierischer Zellen steht meist am Beginn eines jeden wissenschaftlichen Experiments. Erfolg der Versuche und Aussagekraft der Ergebnisse hängen direkt vom Zustand und von der Qualität der kultivierten Zellen ab. Dies setzt voraus, dass die Zellen unter Einhaltung höchster Qualitätsstandards und, wenn möglich, unter Bedingungen der Good Laboratory Practice (GLP) in Kultur gehalten werden (Kap. 15).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Literatur

  • Alston-Roberts C., Barallon R., Bauer S.R., Butler J., Capes-Davis A., Dirks W.G. et al. Cell line misidentification: the beginning of the end. A Report from the American Type Culture Collection Standards Development Organization Workgroup ASN-0002. Nature Rev. Cancer 10: 441–448, 2010.

    Article  Google Scholar 

  • Barallon R., Bauer S.R., Butler J., Capes-Davis A., Dirks W.G. et al. Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues. In Vitro Cell. Dev. Biol. 46: 727–732, 2010.

    Article  Google Scholar 

  • Buehring G.C., Eby E.A. and Eby M.J. Cell line cross-contamination: how aware are mammalian cell culturists of the problem and how to monitor it? In Vitro Cell. Dev. Biol. 40: 211–215, 2004.

    Article  Google Scholar 

  • Cooper-Hannan R., Harbell J.W., Coecke S., Balls M., Bowe G., Cervinka M., Clothier R., Hermann F., Klahm L.K., de Lange J., Liebsch M. and Vanparys P. The principles of Good Laboratory Practice: application to in vitro toxicology studies. ECVAM Workshop Report 37, ATLA 27: 539–577, 1999.

    Google Scholar 

  • Davis J.M. Basic Cell Culture. 2nd Ed., Oxford Univ. Press, 2002.

    Google Scholar 

  • Dirks W.G., Fähnrich S., Estella I.A.J. and Drexler H.G. Short tandem repeat DNA typing provides an international reference standard for authentication of human cell lines. ALTEX 22: 103–109, 2005.

    PubMed  Google Scholar 

  • Dirks W.G., MacLeod R.A.F., Nakamura Y., Kohara A. et al. Cell line cross-contamination initiative: an interactive reference database of STR profiles covering common cancer cell lines. Int. J. Cancer 126: 303–404, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Freshney R.I. Cell line provenance. Cytotechnology 39: 55–67, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Hay R.J. The seed stock concept and quality control for cell lines. Anal. Biochem. 171: 225–237, 1988.

    Article  PubMed  CAS  Google Scholar 

  • Masters J.R.W. Human cancer cell lines: fact and fantasy. Nature Rev. Molec. Cell Biol. 1: 233–236, 2000.

    Article  CAS  Google Scholar 

  • Masters J.R. False cell lines: The problem and a solution. Cytotechnology 39: 69–74, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Masters J.R., Thomson J.A., Daly-Burns B. et al. Short tandem repeat profiling provides an international reference standard for human cell lines. Proc. Natl. Acad. Sci. USA 98: 8012–8017, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Nims R.W., Sykes G., Cottrill K., Ikonomi P. and Elmore E. Short tandem repeat profiling: part of an overall strategy for reducing the frequency of cell misidentification. In Vitro Cell. Dev. Biol. 46: 811–819, 2010.

    Article  Google Scholar 

  • O’Brien S.J. Cell culture forensics. Proc. Natl. Acad. Sci. USA 98: 7656–7658, 2001.

    Article  CAS  Google Scholar 

  • Stacey G.N. and Auerbach J.M. Quality control procedures for stem cell lines. In: Culture of Human Stem Cells (R.I. Freshney, G.N. Stacey and J.M. Auerbach, Eds.). Wiley, 2007.

    Google Scholar 

  • Steube K.G., Grunicke D. and Drexler H.G. Isoenzyme analysis as a rapid method for the examination of the species identity of cell cultures. In Vitro Cell. Dev. Biol. 31: 115–119, 1995.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Gstraunthaler, G., Lindl, T. (2013). Qualitätskontrolle und Cell Banking. In: Zell- und Gewebekultur. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35997-2_14

Download citation

Publish with us

Policies and ethics