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Tolerance, Accumulation, and Detoxification Mechanism of Copper in Elsholtzia splendens

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Book cover Detoxification of Heavy Metals

Part of the book series: Soil Biology ((SOILBIOL,volume 30))

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Abstract

Copper is an essential micronutrient for plant growth and development and yet can be toxic at elevated concentrations. Elsholtzia splendens has been proven to be a Cu-tolerant plant and can remarkably influence the behavior of Cu in root–soil interface by root exudates, rhizosphere bacteria, and arbuscular mycorrhizal fungi. Cu absorbed by plant might in turn interfere with biochemical and physiological process in E. splendens, which as a Cu-tolerant plant has evolved a series of defense strategies against Cu stress: (1) Cu compartmentation: E. splendens could detoxify excess Cu through compartmentation at the tissue and cellular level. (2) Speciation transformation: Histidine-like species probably participated in the transport of Cu from the roots to the shoots. Cu compartmented in the vacuole was not only bound to organic acids such as oxalate acid but also to ligands resembling histidine. Whether phytochelatins play a significant role in Cu detoxification should be further studied. (3) Molecular mechanism: The nonnormalized cDNA library of root tips of E. splendens under Cu stress was constructed. cDNA-AFLP expression profiling showed that a large number of genes in E. splendens were either up- or downregulated upon Cu treatment. Besides, a metallothionein gene was isolated from E. splendens and its function was validated by generating the recombinant E. coli cells and transgenic tobacco plants. The comparative proteomics study suggested that redirection of root cellular metabolism and redox homeostasis might be important survival mechanisms of E. splendens upon Cu stress. All these works lay a solid foundation for establishing engineering plants hyperaccumulating Cu in the future.

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Authors and Affiliations

  1. Institute of Environmental Science and Technology, Zhejiang University, Hangzhou, 310029, China

    Yingxu Chen & Mingge Yu

  2. Ministry of Agriculture, Key Laboratory of Non-point Source Pollution Control, Hangzhou, 310029, China

    Dechao Duan

  3. Department of Environmental Engineering, Huajiachi Campus, Zhejiang University, 268 Kaixuan Road, Hangzhou, Zhejiang, 310029, China

    Dechao Duan

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Correspondence to Yingxu Chen .

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  1. , Inst. Allgemeine Botanik u. Pflanzenphys, Universität Jena, Dornburger Str. 159, Jena, 07743, Germany

    Irena Sherameti

  2. Amity Institute of Microbial Sciences, Amity University Uttar Pradesh, Noida, 201303, Uttar Pradesh, India

    Ajit Varma

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Chen, Y., Yu, M., Duan, D. (2011). Tolerance, Accumulation, and Detoxification Mechanism of Copper in Elsholtzia splendens . In: Sherameti, I., Varma, A. (eds) Detoxification of Heavy Metals. Soil Biology, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21408-0_17

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