Abstract
Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. It is used in vivo for gene therapy, drug therapy and tissue ablation. It has been proposed that measuring the passive electrical properties of electroporated tissues could provide real time feedback on the outcome of the treatment. To generate fundamental data in this sense we have developed a fast spectroscopic impedance analyzer that measures impedance of tissues in the β dispersion region in conjunction with commercial electroporation generators. Here we describe recent results from rat skeletal muscle electroporation (posterior compartment of thigh) and compare them to previously reported results from rat liver. In both studies animals (8+8) were separated into two groups, half of them were subjected electric fields magnitudes of 450 V/cm (ELOW) whereas for the other half magnitudes of 1500 V/cm were applied (EHIGH). The electroporation sequence was the same in all cases: 8 pulses of 100 μs with a period of 100 ms. As expected, tissue conductivity increases in all cases after electroporation, from 9% in the ELOW case for liver to 95% in the EHIGH case for muscle.
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© 2007 Springer-Verlag Berlin Heidelberg
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Ivorra, A., Miller, L., Rubinsky, B. (2007). Electrical impedance measurements during electroporation of rat liver and muscle. In: Scharfetter, H., Merwa, R. (eds) 13th International Conference on Electrical Bioimpedance and the 8th Conference on Electrical Impedance Tomography. IFMBE Proceedings, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-73841-1_36
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DOI: https://doi.org/10.1007/978-3-540-73841-1_36
Publisher Name: Springer, Berlin, Heidelberg
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