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Purification of Pluripotent Stem Cell-Derived Cardiomyocytes for Safe Cardiac Regeneration

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Cardiac Regeneration

Part of the book series: Cardiac and Vascular Biology ((Abbreviated title: Card. vasc. biol.,volume 4))

Abstract

Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), have the potential to differentiate into various cells types and may be used as cell sources for regenerative medicine in the context of various diseases, including severe heart failure. However, one of the biggest hurdles in the use of human PSCs for clinical applications is tumor formation due to contamination with residual tumor-forming cells, primarily undifferentiated PSCs. In addition, hundreds of millions of cardiomyocytes are required for heart repair. Two approaches have been developed for achievement of safer cardiac regenerative therapy using human PSCs: (1) selective elimination of residual tumor-forming cells before cell transplantation and (2) purification of PSC-derived cardiomyocytes. Many methodologies, including genetic and nongenetic modification, have been developed using these strategies. In this chapter, we focus on the current status of selective elimination of residual PSCs and purification of cardiomyocytes for safe stem cell therapy.

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Acknowledgments

The present work was supported by the Highway Program for Realization of Regenerative Medicine from Japan Science and Technology Agency (to K.F.) and SENSHIN Medical Research Foundation (to S.T.).

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Conflict of Interest

The Shugo Tohyama declare that they have no conflict of interest. Keiichi Fukuda is a cofounder of Heartseed Inc.

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This article does not contain any studies with human participants performed by any of the authors.

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Authors and Affiliations

  1. Department of Cardiology, Keio University School of Medicine, Tokyo, Japan

    Shugo Tohyama & Keiichi Fukuda

  2. Department of Organ Fabrication, Keio University School of Medicine, Tokyo, Japan

    Shugo Tohyama

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  1. Shugo Tohyama
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  2. Keiichi Fukuda
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Correspondence to Keiichi Fukuda .

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Editors and Affiliations

  1. Keio University, School of Medicine, Department of Cardiology, Tokyo, Japan

    Masaki Ieda

  2. Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Georg-August University, Göttingen, Germany

    Wolfram-Hubertus Zimmermann

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Tohyama, S., Fukuda, K. (2017). Purification of Pluripotent Stem Cell-Derived Cardiomyocytes for Safe Cardiac Regeneration. In: Ieda, M., Zimmermann, WH. (eds) Cardiac Regeneration. Cardiac and Vascular Biology, vol 4. Springer, Cham. https://doi.org/10.1007/978-3-319-56106-6_8

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