Abstract
Increasing sample numbers for screening and diagnostics using circulating cell-free DNA (ccfDNA) as analyte demands an automated solution for ccfDNA extraction. The efficiency of a new, automated, large volume ccfDNA extraction method was evaluated against a manual reference method. The new kit for automated ccfDNA extraction on the QIAsymphony showed a comparable yield of total ccfDNA from healthy donors as well as a comparable recovery of circulating cancer and fetal DNA. In conclusion, a new kit for automated ccfDNA extraction was established successfully.
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Conflict of Interest
Work presented here was funded by QIAGEN’s R&D Department. A. Wolf and K. Beller are employees of QIAGEN GmbH. S. Groemminger, W. Hofmann and M. Sachse are employees of LifeCodexx AG. J. Fassunke is an employee of Universitätsklinikum Köln.
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© 2016 Springer International Publishing Switzerland
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Wolf, A., Beller, K., Groemminger, S., Hofmann, W., Sachse, M., Fassunke, J. (2016). Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument. In: Gahan, P., Fleischhacker, M., Schmidt, B. (eds) Circulating Nucleic Acids in Serum and Plasma – CNAPS IX. Advances in Experimental Medicine and Biology, vol 924. Springer, Cham. https://doi.org/10.1007/978-3-319-42044-8_33
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DOI: https://doi.org/10.1007/978-3-319-42044-8_33
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Publisher Name: Springer, Cham
Print ISBN: 978-3-319-42042-4
Online ISBN: 978-3-319-42044-8
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