Abstract
Neuronal cultures in vitro plated on the multi-electrode arrays are very promising as an experimental model to study basic principles of learning that can later motivate development of new artificial cognitive architectures. But it is still an open question if patterns of spontaneous activity in neuronal cultures can be interpreted as memory traces and if these traces can be modified in a learning-like manner. We studied experimentally in vitro development of spontaneous bursting activity in neuronal cultures as well as how this activity changes after open or closed loop stimulation. Results demonstrate that bursting activity of neural networks in vitro self-organize into a few number of stereotypic patterns which remain stable over many days. External electrical stimulation increases a number of simultaneously present activity patterns with majority of bursts still classified as belonging to the dominant cluster.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Raichman, N., Ben-Jacob, E.: Identifying repeating motifs in the activation of synchronized bursts in cultured neuronal networks. J. Neurosci. Methods 170(1), 96–110 (2008). doi:10.1016/j.jneumeth.2007.12.020
Shahaf, G., Eytan, D., Gal, A., Kermany, E., Lyakhov, V., Zrenner, C., Marom, S.: Order-based representation in random networks of cortical neurons. PLoS Comput. Biol. 4(11), e1000228 (2008). doi:10.1371/journal.pcbi.1000228
Kermany, E., Gal, A., Lyakhov, V., Meir, R., Marom, S., Eytan, D.: Tradeoffs and constraints on neural representation in networks of cortical neurons. J. Neurosci. Off. J. Soc. Neurosci. 30(28), 9588–9596 (2010). doi:10.1523/JNEUROSCI.0661-10.2010
Xydas, D., Downes, J.H., Spencer, M.C., Hammond, M.W., Nasuto, S.J., Whalley, B.J., Becerra, V.M., Warwick, K.: Revealing ensemble state transition patterns in multi-electrode neuronal recordings using hidden Markov models. IEEE Trans. Neural Syst. Rehabil. Eng. 19(4), 345–355 (2011). doi:10.1109/TNSRE.2011.2157360. (a publication of the IEEE Engineering in Medicine and Biology Society)
Pimashkin, A., Kastalskiy, I., Simonov, A., Koryagina, E., Mukhina, I., Kazantsev, V.: Spiking signatures of spontaneous activity bursts in hippocampal cultures. Front. Comput. Neurosci. 5, 46 (2011). doi:10.3389/fncom.2011.00046
Shahaf, G., Marom, S.: Learning in networks of cortical neurons. J. Neurosci. Off. J. Soc. Neurosci. 21(22), 8782–8788 (2001)
le Feber, J., Stegenga, J., Rutten, W.L.: The effect of slow electrical stimuli to achieve learning in cultured networks of rat cortical neurons. PLoS ONE 5(1), e8871 (2010). doi:10.1371/journal.pone.0008871
Marom, S., Shahaf, G.: Development, learning and memory in large random networks of cortical neurons: lessons beyond anatomy. Q. Rev. Biophys. 35(1), 63–87 (2002)
Li, Y., Zhou, W., Li, X., Zeng, S., Luo, Q.: Dynamics of learning in cultured neuronal networks with antagonists of glutamate receptors. Biophys. J. 93(12), 4151–4158 (2007). doi:10.1529/biophysj.107.111153
Stegenga, J., Le Feber, J., Marani, E., Rutten, W.L.: The effect of learning on bursting. IEEE Trans. Bio-Med. Eng. 56(4), 1220–1227 (2009). doi:10.1109/TBME.2008.2006856
Wagenaar, D.A., Pine, J., Potter, S.M.: An extremely rich repertoire of bursting patterns during the development of cortical cultures. BMC Neurosci. 7, 11 (2006). doi:10.1186/1471-2202-7-11
Sokal, R., Michener, C.: A statistical method for evaluating systematic relationships. Univ. Kans. Sci. Bull. 38(22), 1409–1438 (1958)
Burtsev, M., Lebedev, R.: Evolution of bursts onsets during the development of cortical cultures. Paper presented at the FENS Abstract, vol. 5, pp. 130.12 (2010)
Lebedev, R., Burtsev, M.: Clustering bursts of spontaneous activity of neuronal cultures. In: Proceeding of Neuroinformatics Conference, vol. 1, pp. 296–303. MEPhI Moscow, Russia (2010) (in Russian)
Acknowledgments
This work was supported by the Russian Science Foundation, Grant No. 15-11-30014.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer International Publishing Switzerland
About this paper
Cite this paper
Sokolov, I., Azieva, A., Burtsev, M. (2016). Patterns of Spiking Activity of Neuronal Networks in Vitro as Memory Traces. In: Samsonovich, A., Klimov, V., Rybina, G. (eds) Biologically Inspired Cognitive Architectures (BICA) for Young Scientists . Advances in Intelligent Systems and Computing, vol 449. Springer, Cham. https://doi.org/10.1007/978-3-319-32554-5_31
Download citation
DOI: https://doi.org/10.1007/978-3-319-32554-5_31
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-32553-8
Online ISBN: 978-3-319-32554-5
eBook Packages: EngineeringEngineering (R0)