Abstract
The antibiotic resistance crisis is one of the most pressing public health issues worldwide. Nanoparticles (NPs) can offer a promising solution, since they have antibacterial properties, and can act as carriers for antibiotics and natural antimicrobial compounds. The purpose of this work was to study the antibacterial activity of silver nanoparticles, which are obtained by “green synthesis” from Ocimum araratum extract, against Staphylococcus aureus bacteria, as well as to study their combined action with antibiotic benzylpenicillin. The results show that the antibacterial effect of silver nanoparticles is higher than that of nanoparticles stabilized by the extract on the growth of S. aureus. It has been shown that benzylpenicillin can interact with the allosteric site of penicillin-binding protein 2a. It has also been shown that “green” AgNPs, which include phytocompounds of the extract of O. araratum can enhance the antibacterial effect of benzylpenicillin synergistically.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hemeg, H.: Nanomaterials for alternative antibacterial therapy. Int. J. Nanomed. 10(12), 8211–8225 (2017). https://doi.org/10.2147/ijn.s132163-329
Slavin, Y., Asnis, J., et al.: Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J. Nanobiotechnol. 15, 65 (2017). https://doi.org/10.1186/s12951-017-0308-z
Falagas, M., Kasiakou, S., et al.: Colistin: the revival of polymyxins for the management of multidrug-resistant gram-negative bacterial infections. Clin. Infect. Dis. 40(9), 1333–1341 (2005). https://doi.org/10.1086/429323
Peulen, T.-O., Wilkinson, K.: Diffusion of nanoparticles in a biofilm. Environ. Sci. Technol. 45(8), 3367–3373 (2011). https://doi.org/10.1021/es103450g
Li, P., Pu, X., et al.: FocVel1 influences asexual production, filamentous growth, biofilm formation, and virulence in Fusarium oxysporum f. sp. cucumerinum. Front. Plant. Sci. 6, 312 (2015). https://doi.org/10.3389/fpls.2015.00312
Isiaku, A., Sabri, M., et al.: Biofilm is associated with chronic streptococcal meningoencephalitis in fish. Microb. Pathog. 102, 59–68 (2017). https://doi.org/10.1016/j.micpath.2016.10.029
Fehaid, A., Taniguchi, A.: Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α. Sci. Technol. Adv. Mater. 19(1), 526–534 (2018). https://doi.org/10.1080/14686996.2018.1487761
Mahasenan, K., Molina, R., et al.: Conformational dynamics in penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus, allosteric communication network and enablement of catalysis. J. Am. Chem. Soc. 139(5), 2102–2110 (2017). https://doi.org/10.1021/jacs.6b12565
Bauer, A.: Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45(1), 493–496 (1966). https://doi.org/10.1093/ajcp/45.4_ts.493
Vardapetyan, H., et al.: Antioxidant and antibacterial activities of selected Armenian medicinal plants. JEBAS 2(3), 300–307 (2014)
Ramachandran, S., Kota, P., et al.: Automated minimization of steric clashes in protein structures. Proteins 79(1), 261–270 (2010). https://doi.org/10.1002/prot.22879
Kim, S., Thiessen, P., et al.: PubChem Substance and Compound databases. Nucleic Acids Res. 44(D1), D1202–D1213 (2015). https://doi.org/10.1093/nar/gkv951
Trott, O., Olson, A.: AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J. Comput. Chem. 31(2), 455–461 (2009). https://doi.org/10.1002/jcc.21334
Forli, S., Huey, R., et al.: Computational protein–ligand docking and virtual drug screening with the AutoDock suite. Nat. Protoc. 11(5), 905–919 (2016). https://doi.org/10.1038/nprot.2016.051
Feinstein, W., Brylinski, M.: Calculating an optimal box size for ligand docking and virtual screening against experimental and predicted binding pockets. J. Cheminform. 7(1) (2015). https://doi.org/10.1186/s13321-015-0067-5
McGibbon, R., Beauchamp, K., et al.: MDTraj: a modern open library for the analysis of molecular dynamics trajectories. Biophys. J. 109(8), 1528–1532 (2015)
Laskowski, R., Swindells, M.: LigPlot+: multiple ligand–protein interaction diagrams for drug discovery. J. Chem. Inf. Model. 51(10), 2778–2786 (2011). https://doi.org/10.1021/ci200227u
Conflict of Interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this paper
Cite this paper
Ohanyan, S., Grabski, H., Rshtuni, L., Tiratsuyan, S., Hovhannisyan, A. (2020). Improvement of the Antibacterial Activity of Benzylpenicillin in Combination with Green Silver Nanoparticles Against Staphylococcus aureus. In: Tiginyanu, I., Sontea, V., Railean, S. (eds) 4th International Conference on Nanotechnologies and Biomedical Engineering. ICNBME 2019. IFMBE Proceedings, vol 77. Springer, Cham. https://doi.org/10.1007/978-3-030-31866-6_65
Download citation
DOI: https://doi.org/10.1007/978-3-030-31866-6_65
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-31865-9
Online ISBN: 978-3-030-31866-6
eBook Packages: EngineeringEngineering (R0)