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Production of Specific Flavonoids and Verbascoside in Shoot Cultures of Scutellaria baicalensis

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Book cover Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

Scutellaria baicalensis (Baikal skullcap) has been used for centuries in traditional Chinese medicine (TCM). The root of the Baikal skullcap (Scutellariae baicalensis radix) is also a recognized raw material in European therapeutic practice. It is mentioned in European Pharmacopoeia since 2010 and also in the WHO monograph. This raw material is a rich source of specyfic flavonoids, especially from the group of flavones, such as baicalein, baicalin, wogonin, and wogonoside. These compounds show a number of valuable biological activities.

The aim of the presented research was to propose in vitro cultures of Scutellaria baicalensis as an alternative to the raw material obtained from cultivated plants for providing apart from flavonoids also phenylpropanoid glycosides and phenolic acids. Standard biotechnological treatments, such as a selection of the composition of the basal medium, selection of the concentration and mutual proportions of plant growth regulators, and different types of culture cultivation (solid, agitated and bioreactor’s cultures), were used. In addition, elicitation and feeding with biosynthetic precursors were tested.

The optimization of culturing conditions led to increased accumulation of species-specific flavonoids. Additionally in vitro cultures proved to be a particularly rich source of the phenylpropanoid glycoside - verbascoside.

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Abbreviations

BAP:

6-Benzylaminopurine

DW:

Dry weight

HPLC:

High-Performance Liquid Chromatography

LS:

Linsmaier and Skoog

MS:

Murashige and Skoog

NAA:

α-Naphthaleneacetic acid

PGRs:

Plant Growth Regulators

TCM:

Traditional Chinese Medicine

WHO:

World Health Organization

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Authors and Affiliations

  1. Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Beata Kawka, Inga Kwiecień & Halina Ekiert

Authors
  1. Beata Kawka
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  2. Inga Kwiecień
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  3. Halina Ekiert
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Corresponding author

Correspondence to Inga Kwiecień .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Kawka, B., Kwiecień, I., Ekiert, H. (2019). Production of Specific Flavonoids and Verbascoside in Shoot Cultures of Scutellaria baicalensis. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_7-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_7-1

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  • Print ISBN: 978-3-030-11253-0

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