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High Production of Depsides and Other Phenolic Acids in Different Types of Shoot Cultures of Three Aronias: Aronia Melanocarpa, Aronia Arbutifolia, Aronia × Prunifolia

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Plant Cell and Tissue Differentiation and Secondary Metabolites

Part of the book series: Reference Series in Phytochemistry ((RSP))

Abstract

The search for sources of natural antioxidants has invariably been an attractive scientific and research problem for over a dozen years. One of the subgroups of plant antioxidants includes phenolic acids – derivatives of cinnamic acid and benzoic acid and depsides. These compounds have numerous other valuable directions of biological activity.

The aim of the presented research was broad optimization of the conditions of growing in vitro cultures of three aronias (three chokeberry species), Aronia melanocarpa, A. arbutifolia, and A. × prunifolia, aimed at proposing the conditions most conducive to the production of these compounds. The optimization included testing of basal media, concentrations of plant growth regulators, feeding with biosynthetic precursors of phenolic acids, light conditions (monochromatic and multispectral lights), and different types of shoot cultures – solid and agitated – and also cultures maintained in commercially available bioreactors (RITA and PlantForm).

The use of this strategy, well known in plant biotechnology, allowed us to obtain results potentially of an application nature. The cultures of the three aronias have proved to be a particularly rich source of depsides – chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid, neochlorogenic acid, and rosmarinic acid.

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Abbreviations

BA:

6-Benzyladenine

DW:

Dry weight

HPLC-DAD:

High-performance liquid chromatography with diode-array detection

LS:

Linsmaier and Skoog

MS:

Murashige and Skoog

NAA:

1-Naphthaleneacetic acid

PGRs:

Plant growth regulators

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Jagiellonian University, Collegium Medicum, Kraków, Poland

    Halina Ekiert, Agnieszka Szopa & Paweł Kubica

Authors
  1. Halina Ekiert
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  2. Agnieszka Szopa
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  3. Paweł Kubica
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Corresponding author

Correspondence to Halina Ekiert .

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Editors and Affiliations

  1. Department of Botany, M.L.Sukhadia university, Udaipur, Rajasthan, India

    Kishan Gopal Ramawat

  2. Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Kraków, Poland

    Halina Maria Ekiert

  3. Amarillo, TX, USA

    Shaily Goyal

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Ekiert, H., Szopa, A., Kubica, P. (2019). High Production of Depsides and Other Phenolic Acids in Different Types of Shoot Cultures of Three Aronias: Aronia Melanocarpa, Aronia Arbutifolia, Aronia × Prunifolia. In: Ramawat, K., Ekiert, H., Goyal, S. (eds) Plant Cell and Tissue Differentiation and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-030-11253-0_11-1

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  • DOI: https://doi.org/10.1007/978-3-030-11253-0_11-1

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