Abstract
Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.
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Acknowledgments
This work was supported by the Netherlands Organization for Scientific Research (NWO) Vidi grant 700.56.429. Financial support from EU-COST Action FA0605 is also gratefully acknowledged.
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McLoughlin, F., Testerink, C. (2013). Lipid Affinity Beads: From Identifying New Lipid Binding Proteins to Assessing Their Binding Properties. In: Munnik, T., Heilmann, I. (eds) Plant Lipid Signaling Protocols. Methods in Molecular Biology, vol 1009. Humana, Totowa, NJ. https://doi.org/10.1007/978-1-62703-401-2_25
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DOI: https://doi.org/10.1007/978-1-62703-401-2_25
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Publisher Name: Humana, Totowa, NJ
Print ISBN: 978-1-62703-400-5
Online ISBN: 978-1-62703-401-2
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