Abstract
To study genomic imbalances potentially involved in disease development and/or progression of childhood MDS, array-based comparative genomic hybridization (aCGH) is a helpful tool. Copy number alterations (CNA) of subtle chromosomal regions containing potential candidate genes, e.g., TP53 or RUNX1 can be detected. However, characterizing small and/or heterogeneous tumor subpopulations by high-resolution aCGH within a majority of normal cells is a challenge in MDS and requires validation by independent methods like FISH or quantitative PCR. For the identification of tumor-relevant CNA, the analysis of DNA isolated from purified granulocytes or myeloid populations instead of DNA from whole bone marrow (BM) cells is helpful to overcome some of these limitations.
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Acknowledgments
The authors are grateful to Prof. Brigitte Schlegelberger and Gillian Teicke for critically reading the manuscript and to Dr. Gudrun Göhring for providing the karyogram.
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Tauscher, M., Praulich, I., Steinemann, D. (2013). Array-CGH in Childhood MDS. In: Banerjee, D., Shah, S. (eds) Array Comparative Genomic Hybridization. Methods in Molecular Biology, vol 973. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-281-0_17
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DOI: https://doi.org/10.1007/978-1-62703-281-0_17
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