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Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging

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JAK-STAT Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 967))

Abstract

The ability to observe the dynamic localization of a protein in living cells can provide critical insight to its mode of action and functional molecular interactions. To this purpose, green fluorescent protein (GFP) has served as a powerful tool to tag STAT proteins for microscopic visualization. Live cell imaging with STAT-GFP proteins has contributed to our understanding of signal transduction and the complexities of nuclear transport of STAT proteins. In this report we summarize recent approaches that use GFP-based techniques with live cell imaging to study the mechanisms of STAT nuclear import and export: photoactivation, fluorescence recovery after photobleaching (FRAP), and fluorescence loss in photobleaching (FLIP).

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Acknowledgments

This work was supported by grants from NIH (R56AI095268 and RO1CA122910) and a Carol M. Baldwin Breast Cancer Research Award to NCR.

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Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY, USA

    Velasco Cimica & Nancy C. Reich

Authors
  1. Velasco Cimica
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  2. Nancy C. Reich
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Corresponding author

Correspondence to Nancy C. Reich .

Editor information

Editors and Affiliations

  1. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Sandra E. Nicholson

  2. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Nicos A. Nicola

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Cimica, V., Reich, N.C. (2013). Nuclear Trafficking of STAT Proteins Visualized by Live Cell Imaging. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_14

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  • DOI: https://doi.org/10.1007/978-1-62703-242-1_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-241-4

  • Online ISBN: 978-1-62703-242-1

  • eBook Packages: Springer Protocols

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