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Microfluidic Diagnostics pp 335–347Cite as

Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 949))

Abstract

Conventional detection of disease biomarkers employs techniques such as lateral-flow assays or central laboratory-based enzyme-linked immunosorbent assays (ELISA). Miniaturization and performance improvement of such traditional immunoassays using microfluidic technologies has proved promising in producing rapid, sensitive and automated next-generation immunosensors for quantitative diagnoses in the point-of-care setting. In this article a poly(dimethylsiloxane) (PDMS)-based immunosensor is presented for rapid detection of C-reactive protein. PDMS is selected in part because of the vast popularity of using PDMS as a material for microfluidic devices and in part because of the challenge of obtaining a stable surface coating with PDMS for immunosensing applications. Practical procedures for fabrication, surface modification, and preservation of the microfluidic immuno-chips as well as detailed descriptions of performing the microfluidic heterogeneous assay are presented.

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Author information

Authors and Affiliations

  1. Department of Mechanical, Industrial and Systems Engineering, University of Rhode Island, Kingston, RI, USA

    Peng Li

Authors
  1. Peng Li
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Corresponding author

Correspondence to Peng Li .

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Editors and Affiliations

  1. Key Laboratory for Organic Electronics &, Information Displays (KLOEID), Nanjing Univ. of Posts&Telecommunication, Jia Sheng Hua Yuan, No.2 Building Room 201, Nanjing, 210013, Jiangsu, China, People's Republic

    Gareth Jenkins

  2. Scientific & Medical Communications Cons, avenue Paul Santy 1C, Ecully, 69130, France

    Colin D. Mansfield

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Li, P. (2013). Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers. In: Jenkins, G., Mansfield, C. (eds) Microfluidic Diagnostics. Methods in Molecular Biology, vol 949. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-134-9_21

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  • DOI: https://doi.org/10.1007/978-1-62703-134-9_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-133-2

  • Online ISBN: 978-1-62703-134-9

  • eBook Packages: Springer Protocols

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