Abstract
Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.
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Acknowledgments
This chapter was previously published in the Ph.D. thesis of Benjamin J. Cole, Rapid and dynamic growth of Arabidopsis seedlings in response to changes in light quality: a live imaging study, University of California, San Diego, 2011.
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Cole, B.J., Chory, J. (2012). Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis. In: Normanly, J. (eds) High-Throughput Phenotyping in Plants. Methods in Molecular Biology, vol 918. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-995-2_1
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DOI: https://doi.org/10.1007/978-1-61779-995-2_1
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