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Proteomic Analysis of Skeletal Muscle Tissue Using SELDI-TOF MS: Application to Disuse Atrophy

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SELDI-TOF Mass Spectrometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 818))

Abstract

Skeletal muscle atrophy in response to disuse/unloading is a complex adaptation that involves many components of the muscle tissue. The underlying mechanisms that initiate and control the loss of muscle tissue during this response, especially contractile proteins located within the myofibers, are as yet unclear. One approach capable of distinguishing protein changes specifically associated with disuse/unloading-induced skeletal muscle atrophy is to compare the proteomic profiles of similar muscles between control, unloaded/atrophied, and unloaded/“atrophy-protected” experimental conditions. By utilizing a subtractive proteomic analysis approach, those proteins specifically modulated during the atrophic response can be identified and discriminated from those associated with disuse in general. We here describe the use of SELDI-TOF MS coupled with micro-scale preparative ion-exchange chromatography to detect proteins potentially specifically associated with the atrophic response in rat skeletal muscle.

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Acknowledgments

The author would like to acknowledge Dr. Antonios Kyparos for providing the muscle samples used in this study. This study was facilitated by the existence of a Space Act Agreement between NASA-Johnson Space Center and the University of Houston which allowed access to the SELDI-TOF MS equipment located at NASA-JSC.

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Authors and Affiliations

  1. Department of Health and Human Performance, University of Houston, Houston, TX, USA

    Mark S. F. Clarke

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  1. Mark S. F. Clarke
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Correspondence to Mark S. F. Clarke .

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Editors and Affiliations

  1. The University of Texas, Department of Translational Research, M.D. Anderson Cancer Center, Holcombe Blvd. 1515, Houston, 77030-3722, Texas, USA

    Charlotte H. Clarke

  2. Bio-Rad Laboratories, Great Valley Pkwy. 277, Malvern, 19355, Pennsylvania, USA

    Diane L. Bankert McCarthy

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Clarke, M.S.F. (2012). Proteomic Analysis of Skeletal Muscle Tissue Using SELDI-TOF MS: Application to Disuse Atrophy. In: Clarke, C., McCarthy, D. (eds) SELDI-TOF Mass Spectrometry. Methods in Molecular Biology, vol 818. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-418-6_10

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  • DOI: https://doi.org/10.1007/978-1-61779-418-6_10

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-417-9

  • Online ISBN: 978-1-61779-418-6

  • eBook Packages: Springer Protocols

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