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The Role of Heat Shock Factors in Stress-Induced Transcription

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Molecular Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 787))

Abstract

Heat shock proteins (HSPs) are rapidly induced after stresses, such as heat shock, and accumulate at high concentrations in cells. HSP induction involves a family of heat shock transcription factors that bind the heat shock elements of the HSP genes and mediate transcription in trans. We discuss methods for the study of HSP binding to HSP promoters and the consequent increases in HSP gene expression in vitro and in vivo.

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Acknowledgments

This work was supported by NIH research grants RO-1CA047407, R01CA119045, and RO-1CA094397.

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Authors and Affiliations

  1. Molecular and Cellular Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid & Sheila Schreiner

  2. Urologic Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    Thomas L. Prince

  3. Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Mary Ann Stevenson

  4. Molecular and Cellular Radiation Oncology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA

    Stuart K. Calderwood

Authors
  1. Yue Zhang
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  2. Shiuh-Dih Chou
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  3. Ayesha Murshid
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  4. Thomas L. Prince
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  5. Sheila Schreiner
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  6. Mary Ann Stevenson
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  7. Stuart K. Calderwood
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Corresponding author

Correspondence to Stuart K. Calderwood .

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Editors and Affiliations

  1. Beth Israel Deaconess Medical Center, Department of Radiation Oncology, Harvard Medical School, Brookline Ave 330, Boston, 02215, Massachusetts, USA

    Stuart K. Calderwood

  2. National Cancer Institute, Urologic Oncology Branch, National Institutes of Health, Rockville Pike 9000, Bethesda, 20814, Maryland, USA

    Thomas L. Prince

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Zhang, Y. et al. (2011). The Role of Heat Shock Factors in Stress-Induced Transcription. In: Calderwood, S., Prince, T. (eds) Molecular Chaperones. Methods in Molecular Biology, vol 787. Humana Press. https://doi.org/10.1007/978-1-61779-295-3_2

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  • DOI: https://doi.org/10.1007/978-1-61779-295-3_2

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-294-6

  • Online ISBN: 978-1-61779-295-3

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