Summary
The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation because of its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of one’s antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Gonzalez, R.M., Varnum, S.M., Zangar, R.C. (2008). Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens . In: Wang, F. (eds) Biomarker Methods in Drug Discovery and Development. Methods in Pharmacology and Toxicology™. Humana Press. https://doi.org/10.1007/978-1-59745-463-6_13
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DOI: https://doi.org/10.1007/978-1-59745-463-6_13
Publisher Name: Humana Press
Print ISBN: 978-1-934115-23-7
Online ISBN: 978-1-59745-463-6
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