Overview
- Editors:
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Loïc Faye
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CNRS, Université de Rouen, Mont Saint Aignan, France
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Véronique Gomord
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CNRS, Université de Rouen, Mont Saint Aignan, France
- Fully up-to-date edition offering cutting edge methods for production of recombinant proteins in plants
- Provides readily reproducible protocols for researchers in the field of molecular pharming
- Includes supplementary material: sn.pub/extras
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Table of contents (20 protocols)
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Front Matter
Pages i-xvii
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- Christophe Sourrouille, Brian Marshall, David Liénard, Loïc Faye
Pages 1-23
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- Frank Sainsbury, Li Liu, George P. Lomonossoff
Pages 25-39
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- Marc-André D'Aoust, Pierre-Olivier Lavoie, Julie Belles-Isles, Nicole Bechtold, Michèle Martel, Louis-P. Vézina
Pages 41-50
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- Giuliana Medrano, Michael J. Reidy, Jianyun Liu, Jorge Ayala, Maureen C. Dolan, Carole L. Cramer
Pages 51-67
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- Thomas Rademacher, Elsa Arcalis, Eva Stoger
Pages 69-87
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- Bart Van Droogenbroeck, Kirsten De Wilde, Ann Depicker
Pages 89-101
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- Marta Ayala, Jorge Gavilondo, Meilyn Rodríguez, Alejandro Fuentes, Gil Enríquez, Lincidio Pérez et al.
Pages 103-134
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- David Liénard, Fabien Nogué
Pages 135-144
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- Carole Plasson, Rémy Michel, David Lienard, Claude Saint-Jore-Dupas, Christophe Sourrouille, Ghislaine Grenier de March et al.
Pages 145-161
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- N. Dolendro Singh, Yi Ding, Henry Daniell
Pages 163-192
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- Margarita Torrent, Imma Llop-Tous, M. Dolors Ludevid
Pages 193-208
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- Guy Vancanneyt, Manuel Dubald, Werner Schröder, Jörg Peters, Johan Botterman
Pages 209-221
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- Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann, Patrice Lerouge, José A. Cremata, Véronique Gomord et al.
Pages 239-264
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- Meriem Benchabane, Daniel Rivard, Cécile Girard, Dominique Michaud
Pages 265-273
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- Jose M. Escribano, Daniel M. Perez-Filgueira
Pages 275-287
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- Manfred Gahrtz, Udo Conrad
Pages 289-312
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- Amine M. Badri, Karine Coenen, Louis-Philippe Vaillancourt, Goulet Charles, Dominique Michaud
Pages 313-324
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About this book
Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state-- the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.
Reviews
From the reviews:
“This book presents current methods for the expression of those pharmaceutical proteins in a variety of plant systems. … Each one of its twenty chapters offers objective, clearly written texts including a summary … and some important notes followed by the references. … ‘Recombinant proteins from plants’ is surely a useful tool and a practical and rapid summarized data source with easy procedures on plant molecular pharming for all scientists and related professionals working in this growing field.” (Dominique C. H. Fischer, Brazilian Journal of Pharmaceutical Sciences, 2009)
