Abstract
Antibody reagents that are used for flow cytometry immunophenotyping have traditionally been prepared by combining individual liquid antibody conjugates into mixtures. These cocktails have limited shelf-life, and their preparation is time-consuming and prone to laboratory error. Manufacturers of these reagents, in collaboration with several clinical and research centers, have made advances in constructing dried antibody cocktails which have addressed many of the problems inherent in preparing the liquid cocktails on the lab bench. This chapter discusses methods for the use of dried reagents.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Hammerling JA (2012) A review of medical errors in laboratory diagnostics and where we are today. Lab Med 43(2):41–44
Jamin C, Lann L, Alvarez-Errico D et al (2016) Multi-center harmonization of flow cytometers in the context of the European “PRECISEADS” project. Autoimmun Rev 15(11):1038–1045
van Dongen JJM, Lhermitte L, Böttcher S et al (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9):1908–1975
Van der Velden VHJ, Flores-Montero J, Perez-Andres M et al (2017) Optimization and testing of dried antibody tube: the EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. https://doi.org/10.1016/j.jim.2017.03.011
Glier H, Heijnen I, Hauwel M et al (2017) Standardization of 8-color flow cytometry across different flow cytometer instruments: a feasibility study in clinical laboratories in Switzerland. J Immunol Methods. https://doi.org/10.1016/j.jim.2017.07.013
Hedley BD, Keeney M, Popma J et al (2015) Novel lymphocyte screening tube using dried monoclonal antibody reagents. Cytometry B Clin Cytom 88(6):361–370
Rajab A, Axler O, Leung J et al (2017) Ten-color 15-antibody flow cytometry panel for immunophenotyping of lymphocyte population. Int J Lab Hem 39(Suppl. 1):76–85
Pitoiset F, Cassard L, Soufi E et al (2018) Deep phenotyping of immune cell populations by optimized and standardized flow cytometry analyses. Cytometry A. https://doi.org/10.1002/cyto.a/23570
Maecker HT, McCoy JP, FOCIS Human Immunophenotyping Consortium (2010) A model for harmonizing flow cytometry in clinical trials. Nat Immunol 11(11):975–978
Chan RC, Kotner JS, Chuang CM et al (2017) Stabilization of pre-optimized multicolor cocktails for flow cytometry applications. Cytometry B Clin Cytom 92(6):508–524
Maecker HT, McCoy JP, Nussenblatt R (2012) Standardizing immunophenotyping for the human immunology project. Nat Rev Immunol 12(3):191–200
Finak G, Langweiler M, Jaimes M et al (2016) Standardizing flow cytometry immunophenotyping analysis from the human immunophenotyping consortium. Sci Rep. https://doi.org/10.1038/srep20686
Feher K, Kirsch J, Radbruch A et al (2014) Cell population identification using fluorescence-minus-one controls with a one-class classifying algorithm. Bioinformatics 30(23):3372–3378
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Langweiler, M. (2019). Immunophenotyping Using Dried and Lyophilized Reagents. In: McCoy, Jr, J. (eds) Immunophenotyping. Methods in Molecular Biology, vol 2032. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9650-6_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9650-6_4
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9649-0
Online ISBN: 978-1-4939-9650-6
eBook Packages: Springer Protocols