Abstract
Drosophila is a useful model organism for studying the molecular signatures that define specific muscle types during myogenesis. It possesses significant genetic conservation with humans for muscle disease causing genes and a lack of redundancy that simplifies functional analysis. Traditional molecular methods can be utilized to understand muscle developmental processes such as Western blots, in situ hybridizations, RT-PCR and RNAseq, to name a few. However, one challenge for these molecular methods is the ability to dissect different muscle types. In this protocol we describe some useful techniques for extracting muscles from the pupal and adult stages of development using flight and jump muscles as an example.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Bönnemann CG, Laing NG (2004) Myopathies resulting from mutations in sarcomeric proteins. Curr Opin Neurol 17(5):529–537
D’Amico A, Bertini E (2008) Congenital myopathies. Curr Neurol Neurosci Rep 8:73–79
Schiaffino S, Reggiani C (2011) Fiber types in mammalian skeletal muscles. Physiol Rev 91(4):1447–1531
Ariano MA, Armstrong RB, Edgerton VR (1973) Hindlimb muscle fiber populations of five mammals. J Histochem Cytochem 21(1):51–55
Smith RS, Ovalle WK Jr (1973) Varieties of fast and slow extrafusal muscle fibres in amphibian hind limb muscles. J Anat 116(Pt 1):1–24
Gleeson TT, Putnam RW, Bennett AF (1980) Histochemical, enzymatic, and contractile properties of skeletal muscle fibers in the lizard Dipsosaurus dorsalis. J Exp Zool 214(3):293–302
Thorstensson A, Grimby G, Karlsson J (1976) Force-velocity relations and fiber composition in human knee extensor muscles. J Appl Physiol 40(1):12–16
Harridge SD et al (1996) Whole-muscle and single-fibre contractile properties and myosin heavy chain isoforms in humans. Pflugers Arch 432(5):913–920
Hoppeler H et al (1973) The ultrastructure of the normal human skeletal muscle. A morphometric analysis on untrained men, women and well-trained orienteers. Pflugers Arch 344(3):217–232
Herbison GJ, Jaweed MM, Ditunno JF (1982) Muscle fiber types. Arch Phys Med Rehabil 63(5):227–230
Rossi AC et al (2010) Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles. J Physiol 588(Pt 2):353–364
Korfage JA, Van Eijden TM (2003) Myosin heavy-chain isoform composition of human single jaw-muscle fibers. J Dent Res 82(6):481–485
Costill DL et al (1976) Skeletal muscle enzymes and fiber composition in male and female track athletes. J Appl Physiol 40(2):149–154
Agudelo LZ et al (2014) Skeletal muscle PGC-1alpha1 modulates kynurenine metabolism and mediates resilience to stress-induced depression. Cell 159(1):33–45
Butler-Browne GS, Whalen RG (1984) Myosin isozyme transitions occurring during the postnatal development of the rat soleus muscle. Dev Biol 102(2):324–334
Ciciliot S et al (2013) Muscle type and fiber type specificity in muscle wasting. Int J Biochem Cell Biol 45(10):2191–2199
Lexell J (1995) Human aging, muscle mass, and fiber type composition. J Gerontol A Biol Sci Med Sci 50:11–16
Oberbach A et al (2006) Altered fiber distribution and fiber-specific glycolytic and oxidative enzyme activity in skeletal muscle of patients with type 2 diabetes. Diabetes Care 29(4):895–900
Tanner CJ et al (2002) Muscle fiber type is associated with obesity and weight loss. Am J Physiol Endocrinol Metab 282(6):E1191–E1196
Bryantsev AL et al (2012) Differential requirements for myocyte enhancer factor-2 during adult myogenesis in Drosophila. Dev Biol 361(2):191–207
Oas ST, Bryantsev AL, Cripps RM (2014) Arrest is a regulator of fiber-specific alternative splicing in the indirect flight muscles of Drosophila. J Cell Biol 206(7):895–908
Chechenova MB et al (2017) Functional redundancy and non-redundancy between two troponin C isoforms in Drosophila adult muscles. Mol Biol Cell 28:760
Chechenova MB, Bryantsev AL, Cripps RM (2013) The Drosophila Z-disc protein Z(210) is an adult muscle isoform of Zasp52, which is required for normal myofibril organization in indirect flight muscles. J Biol Chem 288(6):3718–3726
Bryantsev AL et al (2012) Extradenticle and homothorax control adult muscle fiber identity in Drosophila. Dev Cell 23(3):664–673
Cripps RM, Sparrow JC (1992) Polymorphism in a Drosophila indirect flight muscle-specific tropomyosin isozyme does not affect flight ability. Biochem Genet 30:159
Acknowledgments
Research at UNM is supported by grants from NIH/NIGMS: COBRE Center for Evolutionary and Theoretical Immunology (P30GM110907), the NIH (GM061738 and GM124498) and NSF (1518073) to R.M. Cripps.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bryantsev, A.L., Castillo, L., Oas, S.T., Chechenova, M.B., Dohn, T.E., Lovato, T.L. (2019). Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_16
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8897-6_16
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8896-9
Online ISBN: 978-1-4939-8897-6
eBook Packages: Springer Protocols