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Somatic Stem Cells pp 55–68Cite as

Identification and Isolation of Mice and Human Hematopoietic Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1842))

Abstract

Hematopoietic stem cells (HSCs) are multipotent cells capable of differentiating into all types of blood cells. The important feature of the HSCs is their ability to repopulate the complete blood cells after BM ablation. For clinical application, cord blood derived HSCs and G-CSF mobilized peripheral blood HSCs are good alternative to bone marrow HSCs. For immunological and hematological studies the obvious choice of model organism is Mouse. Therefore, understanding HSCs in murine model is important. In this chapter, we describe the common/currently used methods to isolate and identify human and mouse HSCs.

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Author notes

  1. Bijender Kumar, Srideshikan Sargur Madabushi contributed equally to this work.

Authors and Affiliations

  1. Department of Radiation Oncology, City of Hope Medical Center, Duarte, CA, USA

    Bijender Kumar & Srideshikan Sargur Madabushi

Authors
  1. Bijender Kumar
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  2. Srideshikan Sargur Madabushi
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Corresponding author

Correspondence to Bijender Kumar .

Editor information

Editors and Affiliations

  1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, USA

    Pranela Rameshwar

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Kumar, B., Madabushi, S.S. (2018). Identification and Isolation of Mice and Human Hematopoietic Stem Cells. In: Singh, S., Rameshwar, P. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 1842. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8697-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8697-2_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8696-5

  • Online ISBN: 978-1-4939-8697-2

  • eBook Packages: Springer Protocols

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