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Receptor-Receptor Interactions in the Central Nervous System pp 317–328Cite as

Unraveling the Functions of Endogenous Receptor Oligomers in the Brain Using Interfering Peptide: The Example of D1R/NMDAR Heteromers

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Part of the book series: Neuromethods ((NM,volume 140))

Abstract

Decoding signaling pathways in different brain structures is crucial to develop pharmacological strategies for neurological diseases. In this perspective, the targeting of receptors by selective ligands is one of the classical therapeutic strategies. Nonetheless, this approach often results in a decrease of efficiency over time and deleterious side effects because physiological functions can be affected. An emerging concept has been to target mechanisms that fine-tune receptor signaling, such as heteromerization, the process by which physical receptor–receptor interaction at the membrane allows the reciprocal modulation of receptors’ signaling. Because of the central role of the synergistic transmission mediated by dopamine (DA) and glutamate (Glu) in brain physiology and pathophysiology, heteromerization between DA and Glu receptors has received a lot of attention. However, the study of endogenous heteromers has been challenging because of the lack of appropriate tools. Over the last years, progress has been made in the development of techniques to study their expression in the brain, regulation and function. In this chapter, we provide a methodological framework for the design and use of interfering peptides to study endogenous receptor oligomers through the example of the dopamine type 1 receptor (D1R) and the GluN1 subunit of NMDA receptor heteromers.

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Author information

Authors and Affiliations

  1. UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris-Seine, Institut de Biologie Paris Seine (NPS-IBPS), Sorbonne Universités, Paris, France

    Andry Andrianarivelo, Estefani Saint-Jour & Peter Vanhoutte

  2. UPMC Univ Paris 06, IPAL, CNRS, Sorbonnes Universités, Paris, France

    Andry Andrianarivelo, Estefani Saint-Jour & Peter Vanhoutte

  3. Nutrition and Integrative Neurobiology, INRA UMR-1286, Bordeaux, France

    Pierre Trifilieff

  4. University of Bordeaux, Bordeaux, France

    Pierre Trifilieff

  5. Neuronal Signaling and Gene Regulation, UMR-S 1130/UMR8246, Université Pierre et Marie Curie-Paris 06, Paris, France

    Peter Vanhoutte

Authors
  1. Andry Andrianarivelo
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  2. Estefani Saint-Jour
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  3. Pierre Trifilieff
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  4. Peter Vanhoutte
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Corresponding author

Correspondence to Peter Vanhoutte .

Editor information

Editors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    KJELL FUXE

  2. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

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Andrianarivelo, A., Saint-Jour, E., Trifilieff, P., Vanhoutte, P. (2018). Unraveling the Functions of Endogenous Receptor Oligomers in the Brain Using Interfering Peptide: The Example of D1R/NMDAR Heteromers. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8576-0_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8575-3

  • Online ISBN: 978-1-4939-8576-0

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