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Virus-Derived Nanoparticles for Advanced Technologies pp 533–552Cite as

Dual-Functionalized Virus–Gold Nanoparticle Clusters for Biosensing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1776))

Abstract

Metallic nanoscale 3D architectures concentrate electromagnetic energy at precise spatial locations to enable sensing and photocatalysis applications. We have developed solution-based methods to reproducibly fabricate 3D gold nanostructures useful as efficient surface-enhanced Raman spectroscopy (SERS) biosensors. Virus capsids were recruited as templates to assemble gold nanoparticles on their surfaces at well-defined locations to prepare the nanoscale 3D structures. Cowpea mosaic virus (CPMV) and its variants were selected as specific templates due to their high symmetry, scalability, and stability, which have proven useful in materials science applications. While the methods described herein were optimized for the CPMV capsids, they also provide a useful starting point for researchers who are working toward the nanoassembly of metal nanoparticles on other protein scaffolds.

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Acknowledgments

This work was supported by the Office of Naval Research 6.1 base funds. We thank J. Phelps and J. E. Johnson for BC-CPMV samples and Y. Meshcheriakova and G. Lomonossoff for VLP samples.

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Author notes

  1. Carissa M. Soto

    Present address: Materials and Applications Division, US Bureau of Engraving and Printing, Washington, DC, USA

Authors and Affiliations

  1. Center for Bio/Molecular Science and Engineering, US Naval Research Laboratory, Washington, DC, USA

    Carissa M. Soto & Walter J. Dressick

Authors
  1. Carissa M. Soto
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  2. Walter J. Dressick
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Corresponding author

Correspondence to Carissa M. Soto .

Editor information

Editors and Affiliations

  1. Institute of Biomaterials and Biomolecular Systems, University of Stuttgart, Stuttgart, Germany

    Christina Wege

  2. Department of Biological Chemistry, John Innes Centre, Norwich, United Kingdom

    George P. Lomonossoff

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Soto, C.M., Dressick, W.J. (2018). Dual-Functionalized Virus–Gold Nanoparticle Clusters for Biosensing. In: Wege, C., Lomonossoff, G. (eds) Virus-Derived Nanoparticles for Advanced Technologies. Methods in Molecular Biology, vol 1776. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7808-3_34

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  • DOI: https://doi.org/10.1007/978-1-4939-7808-3_34

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7806-9

  • Online ISBN: 978-1-4939-7808-3

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