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Neurotrophic Factors pp 423–432Cite as

Recreating Intestinal Peristalsis in the Petri Dish

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

Here we describe a culture technique of cells dissociated from the external muscularis of the guinea pig small intestine, which allows us to maintain all the elements involved in the intestinal peristaltic reflex. After a few days in culture, these cells reorganize to form a small group of cells that permit the generation of pacemaker activity, spontaneous contractions, and the development of inhibitory and excitatory junction potentials in the petri dish, all elements involved in the peristaltic reflex. Therefore, these co-cultures are suitable to study the cellular and molecular aspects related to the development, maintenance, and modulation of motor intestinal functions.

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Acknowledgments

This work was supported by CONACYT, México (Project No. 81409).

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Authors and Affiliations

  1. IPICYT/División de Biología Molecular, San Luis Potosí, Mexico

    Rosa Espinosa-Luna & Carlos Barajas-López

  2. Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico

    Alma R. Barajas-Espinosa & Fernando Ochoa-Cortez

Authors
  1. Rosa Espinosa-Luna
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  2. Alma R. Barajas-Espinosa
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  3. Fernando Ochoa-Cortez
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  4. Carlos Barajas-López
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Corresponding author

Correspondence to Carlos Barajas-López .

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Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Espinosa-Luna, R., Barajas-Espinosa, A.R., Ochoa-Cortez, F., Barajas-López, C. (2018). Recreating Intestinal Peristalsis in the Petri Dish. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_34

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_34

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

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