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Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes

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Bacteriophages

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1681))

Abstract

The capacity of transposable elements to insert into the genomes has been harnessed during the past decades to various in vitro and in vivo applications. This chapter describes in detail the general protocols and principles applicable for the Mu in vitro transposition reaction as well as the assembly of DNA transposition complexes that can be electroporated into bacterial cells to accomplish efficient gene delivery. These techniques with their modifications potentiate various gene and genome modification applications, which are discussed briefly here, and the reader is referred to the original publications for further details.

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Acknowledgments

This work was supported by the Academy of Finland (Grant 251168).

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Authors and Affiliations

  1. Division of Genetics and Physiology, Department of Biology, University of Turku, Turku, FI-20014, Finland

    Saija Haapa-Paananen & Harri Savilahti

Authors
  1. Saija Haapa-Paananen
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  2. Harri Savilahti
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Corresponding author

Correspondence to Harri Savilahti .

Editor information

Editors and Affiliations

  1. Department Infection, Immunity & Inflammation, University of Leicester School of Medicine, Leicester, United Kingdom

    Martha R.J. Clokie

  2. University of Guelph, Guelph, Ontario, Canada

    Andrew M. Kropinski

  3. KU Leuven, Laboratory of Gene Technology, Leuven, Belgium

    Rob Lavigne

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Haapa-Paananen, S., Savilahti, H. (2018). Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes. In: Clokie, M., Kropinski, A., Lavigne, R. (eds) Bacteriophages. Methods in Molecular Biology, vol 1681. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7343-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7343-9_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7341-5

  • Online ISBN: 978-1-4939-7343-9

  • eBook Packages: Springer Protocols

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