Abstract
Prostanoids and hydroxy fatty acids derived from polyunsaturated fatty acids are widely recognized as mediators of inflammatory and homeostatic processes in the body. Accurate identification and quantification of these lipid mediators can aid in understanding of lipid metabolism in different tissues under different physiological and pathological conditions, potentially leading to biomarker discovery and identification of therapeutic targets, as well as providing support for the efficacy of novel therapies. The protocol outlined here describes the extraction of these lipids from a range of biological samples (including solid tissues, biological fluids, and cell culture media), their semi-purification by solid-phase extraction, and their analysis by reverse-phase ultra-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. This provides highly sensitive and specific quantitative analysis of bioactive lipid species.
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Acknowledgments
We acknowledge the excellent technical support provided by Neil O’Hara, Manchester Pharmacy School, The University of Manchester.
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Kendall, A.C., Nicolaou, A. (2017). Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids. In: Wood, P. (eds) Lipidomics. Neuromethods, vol 125. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6946-3_15
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DOI: https://doi.org/10.1007/978-1-4939-6946-3_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6944-9
Online ISBN: 978-1-4939-6946-3
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