Abstract
Synthetic derivatization of hormonally active brassinosteroids (BRs) can provide useful small molecule tools to probe BR signaling pathways, such as fluorescent analogs. However, most biologically active BRs are not suitable for direct chemical conjugation techniques because their derivatization typically requires extensive synthetic work and chemistry expertise. Here, we describe an operationally simple, two-step procedure to prepare and purify an Alexa Fluor 647-castasterone (AFCS) from commercially available materials. The reported strategy is also amenable to the introduction of various other amine-based labeling groups.
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Acknowledgments
NGI is indebted to the Odysseus program of the Research Foundation-Flanders for a postdoctoral fellowship.
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Winne, J.M., Irani, N.G., Van den Begin, J., Madder, A. (2017). Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye. In: Russinova, E., Caño-Delgado, A. (eds) Brassinosteroids. Methods in Molecular Biology, vol 1564. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6813-8_2
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DOI: https://doi.org/10.1007/978-1-4939-6813-8_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6811-4
Online ISBN: 978-1-4939-6813-8
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