Abstract
Adoptive transfer of T lymphocytes is a useful technique to characterize the role of the immune system in hypertension and vascular disease. Here we describe as an example the isolation of splenic T regulatory cells from donor mice processed to obtain a single cell suspension, followed by negative and positive selection to obtain CD4+ T cells and CD4+CD25+ Treg cells, respectively. Treg cells can be subsequently transferred to recipient animals.
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Acknowledgments
We are grateful to Christian Young for excellent technical assistance and suggestions. The work of the authors was supported by Canadian Institutes of Health Research grants 82790 and 102606, a Canada Research Chair on Hypertension and Vascular Research from Canadian Institutes of Health Research/Government of Canada, and the Canada Fund for Innovation (all to E.L.S.). K.K.M is supported by CIHR grant 115000 and is a Junior 2 Chercheur Boursier of the FRQ-S.
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Barhoumi, T., Paradis, P., Mann, K.K., Schiffrin, E.L. (2017). Isolation of Immune Cells for Adoptive Transfer. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_25
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DOI: https://doi.org/10.1007/978-1-4939-6625-7_25
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