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Isolation and Characterization of Circulating Microparticles by Flow Cytometry

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Hypertension

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

Microparticles are small fragments (0.1–1.0 μm) of cellular membrane which are shed by cells under conditions of stress. As levels of circulating microparticles are elevated in disease, there has been significant interest in their assessment and quantification under pathological conditions. Here we describe a protocol for the isolation of microparticles from plasma samples and their characterization/quantification by flow cytometry. This assay has been employed for the assessment of microparticle levels in both human and animal plasma and may also be modified for the characterization of microparticles from culture media or from other biological samples (i.e., urine).

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Acknowledgments

D.B. is supported by a Kidney Research Scientist Core Education and National Training Program (KRESCENT) New Investigator Award.

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Authors and Affiliations

  1. Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, 451 Smyth Rd., Ottawa, ON, Canada, K1H 8M5

    Dylan Burger Ph.D.

  2. Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada

    Paul Oleynik

Authors
  1. Dylan Burger Ph.D.
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  2. Paul Oleynik
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Corresponding author

Correspondence to Dylan Burger Ph.D. .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

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Burger, D., Oleynik, P. (2017). Isolation and Characterization of Circulating Microparticles by Flow Cytometry. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_21

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

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