Abstract
Chemotherapy is one of the major therapeutic interventions in oncology. Despite numerous advances and intensive research, a large number of patients acquire multidrug resistance (MDR) and no longer respond to chemotherapy. Efflux transporters play a predominant role in mediating MDR. Cellular accumulation (uptake) and permeability studies serve as invaluable methods to detect drug efflux/transport mechanism. These methods are generally performed on transfected cells (e.g., MDCKII-MDR1, MDCKII-MRP2, and MDCKII-BCRP) or cells expressing high amount of intrinsic efflux transporters (Caco-2) utilizing specific inhibitors as positive controls. This chapter presents a method of performing uptake and permeability studies, including the preparation of various buffers required for the study.
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Vadlapatla, R.K., Pal, D., Mitra, A.K. (2016). Uptake and Permeability Studies to Delineate the Role of Efflux Transporters. In: Rueff, J., Rodrigues, A. (eds) Cancer Drug Resistance. Methods in Molecular Biology, vol 1395. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3347-1_5
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DOI: https://doi.org/10.1007/978-1-4939-3347-1_5
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3345-7
Online ISBN: 978-1-4939-3347-1
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