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Analysis by Flow Cytometry of B-Cell Activation and Antibody Responses Induced by Toll-Like Receptors

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Toll-Like Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1390))

Abstract

Toll-like receptors (TLRs) are expressed in B lymphocytes and contribute to B-cell activation, antibody responses, and their maturation. TLR stimulation of mouse B cells induces class switch DNA recombination (CSR) to isotypes specified by cytokines, and also induces formation of IgM+ as well as class-switched plasma cells. B-cell receptor (BCR) signaling, while on its own inducing limited B-cell proliferation and no CSR, can enhance CSR driven by TLRs. Particular synergistic or antagonistic interactions among TLR pathways, BCR, and cytokine signaling can have important consequences for B-cell activation, CSR, and plasma cell formation. This chapter outlines protocols for the induction and analysis of B-cell activation and antibody production by TLRs with or without other stimuli.

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  1. Department of Pharmaceutical Sciences, University of California, Irvine, CA, 92697, USA

    Egest J. Pone

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  1. Hudson Institute of Medical Research, Monash University Faculty of Medicine, Clayton, Victoria, Australia

    Claire E. McCoy

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Pone, E.J. (2016). Analysis by Flow Cytometry of B-Cell Activation and Antibody Responses Induced by Toll-Like Receptors. In: McCoy, C. (eds) Toll-Like Receptors. Methods in Molecular Biology, vol 1390. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3335-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3335-8_15

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